University of Khartoum

Antimicrobial and Anti-inflammatory Activity of Plants Indiginous to Sudan

Antimicrobial and Anti-inflammatory Activity of Plants Indiginous to Sudan

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Title: Antimicrobial and Anti-inflammatory Activity of Plants Indiginous to Sudan
Author: Ismaiel, Sarah Mustafa Osman
Abstract: Atotal of 18 plant extracts belonging to 5 Sudanese medicinal plants, distributed among 5 families were screened for their antimicrobial and/or anti inflammatory activity. The plants are: 1. Acacia nilotica (L.)willd , F. Fabaceae (babul) 2. Hibiscus sabdariffa (L). ,F.Malvaceae (Roselle) 3. Hordeum vulgare(L)., F.Poaceae (Barley) 4. Salvadora persica (L). F.Salvadoraceae, (tooth brush tree) 5. Ziziphus spina-christi (L.)willd. ,F.Rhamnaceae (christs Thorn) For the antimicrobial activity ,the technique used was the cup plate agar diffusion method, using different solvents: chloroform, methanol, and water, they were tested against six microorganisms, four standard bacterial organisms one gram positive bacteria namely: (Staphylococcus aureus) and three gram negative bacteria (Eschrichia coli, , Proteus vulgaris and Pseudomonas aeruginosa) and two fungal organisms namely: (Aspergillus niger and Candida albicans) Out of the 18 extracts, 8 extracts (44.4%) exhibited inhibitory activity against one or more of the six tested microorganisms. Out ofthe 8 extracts showing antimicrobial inhibitory activity, 3(37.5%) were inhibitory to the six tested micro organisms. Eschrichia coli was the most susceptible microorganisms to the extracts, while Pseudomonas aerginosa showed the least susceptibility. The minimum inhibitory concentrations(MICs) of the most active extracts against standard organisms were determined. using agar dilution method. The antimicrobial activity of five reference drugs were determined against the six tested microorganisms and compared to the antimicrobial activity of the tested plant extracts. The most active extracs were four belonging to two plants :Acacia nilotica and Hibiscus sabdariffa in metahol and water ,then the MICs and the preliminary Phytochemical screening.was done. For the antiinflammatory activity , the method used was carrageenan induced oedema in albino rats. Using diclofenac sodium as reference anti inflammatory drug. The solvent was sodium carbonate. Out of 7 extracts 3 extracts (43%) exhibited antiinflammatory activity and decrease the oedema from the paw of the albino rats. The percent inhibition in the paw volume was determined for the 7 extracts after different interval and the data was estimated graphically. The most active extracts were three belonging to 3 plants. Acacia nilotica showed the highest antimicrobial activity , Hibiscus sabdariffa also revealed a good antimicrobial activity and Salvadora persica showed mild antimicrobial activity, MeOH/H2O extracts showed higher activity than chloroform ones, the other plants didn’t show any antimicrobial activity. Ziziphus spina-christi showed the highest anti inflammatory avtivity and Hordeum vulgare also showed mild antiinflammatory response while the other plants didn’t show any antiinflammatory activity. The preliminary Phytochemical screening of the 5 plants indicated that they contain many important chemical constituents including: saponins, tannins, sterols, triterpenoids, alkaloids, flavonoids, coumarins and cyanogenic glycosides.
URI: http://khartoumspace.uofk.edu/123456789/23938


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