University of Khartoum

Phenotypic and Genotypic Analysis of Multi-drug Resistance Mycobacterium Tuberculosis Isolates from Sudanese Patients

Phenotypic and Genotypic Analysis of Multi-drug Resistance Mycobacterium Tuberculosis Isolates from Sudanese Patients

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Title: Phenotypic and Genotypic Analysis of Multi-drug Resistance Mycobacterium Tuberculosis Isolates from Sudanese Patients
Author: Sabeel, Solima Mohamed Abdalla
Abstract: Background: Currently, mutations in rpoB, KatG, rrs genes and inhA promoter were considered to be involved in conferring resistance to first line anti-tuberculosis drugs such as rifampicin, isoniazid and streptomycin in Mycobacterium tuberculosis (MTB) as cross-sectional descriptive study conducted in Abo-Anga hospital for chest disease. The objective of the study was to characterize multi-drug resistance MTB Methods: A hundred sputum samples were collected from Sudanese pulmonary TB patients. These samples were cultured in Lôwenstein-Jonson media (LJ), followed by proportional method of drug susceptibility test which carried out on LJ containing first line drugs. Multiplex PCR of rpoB, KatG genes and inhA promoter had been conducted then rrs genes were amplified by conventional PCR. The sequences of the PCR product were compared with known rrs gene sequences in the Gene Bank database by multiple sequence alignment tools. Results: The occurrences of MDR were 20% (15/75), 14.7% among old cases and 5.3% among newly diagnosed cases. Sixty two percent isolates were streptomycin resistant, 34% were rifampicin resistant, 20% isolates were euthambutol resistant and 34% isolates were isoniazid resistant. Polymorphisms in rrs gene were classified into seven clusters (containing 1–4 isolates per cluster). No mutation affecting the 530 loop were found, 23% of mutations were found on 912 loop and 23% of streptomycin-resistant isolates revealed single point mutations at position 222 (C→A). Conclusion: Mutations in the rrs gene were detected in 62% of streptomycin-resistant M. tuberculosis. The presence of the C222A substitution in rrs gene could be considered as streptomycin-resistance diagnostic marker.
URI: http://khartoumspace.uofk.edu/123456789/24473


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