University of Khartoum

The Antiurolithic and Nephroprotective Effects of Aquous Extracts of Ambrosia maritima and Blepharis Iinariifolia

The Antiurolithic and Nephroprotective Effects of Aquous Extracts of Ambrosia maritima and Blepharis Iinariifolia

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Title: The Antiurolithic and Nephroprotective Effects of Aquous Extracts of Ambrosia maritima and Blepharis Iinariifolia
Author: Babiker, Thowiba Ahmed Ibrahim
Abstract: The antiuroliathatic and nephroprotective activities of two medicinal plants aqueous extracts of Ambrosia maritima and Blepharis linariifolia were studied in Albino rats (60-100 g). Uroliathasis was induced in rats by intraperitoneally injected with 7mg/Kg body weight sodium oxalate. Rats that showed signs of urolithiasis treated orally with the aqueous extract of the whole plants of A. maritima and B. linariifolia at doses 250mg/Kg body weight and 500mg/Kg body weight for 28 days for each group of animals. The positive control group received standard drug Thiazide (150 mg/kg), while the negative control group received distilled water. Blood samples were collected on days 14th and 28th of the treatment. Plasma was separated for determination of potassium, magnesium, creatinine and urea levels. Kidney tissue samples were collected at the end of experiment for histopathological investigation. Nephrotoxicity was induced in rats by intraperitoneal injection of Gentamicine 80mg/Kg daily for 8 days. Concurrently with two doses of 200mg/Kg body weight and 400mg/Kg body weight of aqueous extract of the whole plants of A. maritima and B. linariifolia were administered orally for 8 days also. The control group received distilled water. Plasma potassium, magnesium, creatinine and urea levels were determined before and after treatment. Kidney tissue samples were collected at the end of experiment for histopathological investigation. Statistical analysis was carried using SPSS. Values were considered significant at (P<0.05). In the urolithiaic group of rats, the dose of 250mg/kg Ambrosia maritirna extract caused a significant (P<0.05) decrease in Mg (0.83mg/dl) and K (6.32mg/dl) levels compared to the negative control. While after 28 days, urea (42.26mg/dl) and Mg (0.78mg/dl) were significantly (P< 0.05) decreased compared to the negative control. However, creatinine level (0.98mg/dl) remained in the normal rang. At the dose of 500 mg/kg Ambrosia maritima aqueous extract caused an increase in K (8.93mg/dl) and urea (47.00mg/dl) levels after 14 and 28 days. Aqueous extract of Blepharis linaritfolia (250mg/kg) after 14 days caused a significant (P <0.05) decrease in K (6.50mg/dl), although there was an insignificant decrease in creatinine level (0.95mg/dl) at the same time compared to the control group. Treatment for 28 days showed significant (P<0.05) decrease in creatinine level (0.98mg/dl). A dose of 500 mg/kg Blepharis linariifolia caused a significant (P<0.05) decrease in K (3.68mg/dl), Mg (2.68mg/dl), urea (31.2mg/dl) and creatinine levels (0.86mg/dl) after 14 and 28 days. In nephroprotective model, 200 mg/kg and 400mg/kg of Ambrosia maritirna extract showed an increase in K (5.38mg/dl and 5.87mg/dl), urea (63.67mg/dl and56.58mg/dl) and creatinine levels (2.18mg/dl and 1.87mg/dl) after 8 days compared to control. While Blepharis iinariifolia (200mg/kg) after 8 days caused a significant (p<0.05) decrease in urea level (38.48mg/dl). The results of present study showed that Ambrosia maritima possess antilithiatic effect at dose 250mg/kg with signs of toxicity at dose 500 mg/kg. However, nephroprotective effect was not observed with Ambrosia maritima. Blepharis linariifolia exhibited antilithiatic effect in dose range (200-400 mg/kg) and nephroprotictive effect at dose 200mg/kg.
Description: 90 Pages
URI: http://khartoumspace.uofk.edu/123456789/24486


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