University of Khartoum

Antibacterial Activity of Clove oil Against Some Microorganism at Khartoum state

Antibacterial Activity of Clove oil Against Some Microorganism at Khartoum state

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Title: Antibacterial Activity of Clove oil Against Some Microorganism at Khartoum state
Author: Fagere, Zahra Osman Osman
Abstract: Background: Microorganisms have increased and that may cause some health problems, so it has become necessary to find out new antibacterial agents. To Prevent Pathogenic microorganisms and spoilage of food. This is a descriptive cross- sectional study aiming at evaluating the antibacterial activity of the essential oil of Syzygium aromaticum against selected bacteria. Khartoum State-Sudan. Methodology: The essential oil of S.aromaticum was screened for in vitro antibacterial activity against four standard bacteria Two Gram positive :Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923). And two Gram negative: Eschericia coli (ATCC 25922), and Psuedomonas aeruginosa (ATCC 27853), using the cup plate agar diffusion method, and the micro-dilution method. Results: The essential oil of Syzygium aromaticum dissolved in methanol (1:10) showed high activity (28 & 22 mm) against Gram positive bacteria (S. aureus & B. subtilis) and (26 & 21 mm) against Gram negative bacteria (E. coli & P. aeruginosa).The MIC of the essential oil of S. aromaticum against Gram positive (Bacillus subtilis and Staphylococcus aureus) was (0.78 mg/ml), and against the Gram negative (Escherichia coli and Pseudomonas aeruginosa ) was (1.5 mg/ml). The MBC of the essential oil of S. aromaticum against (Bacillus subtilis ,Staphylococcu aureus and Escherichia coli was (3.125 mg/ml) and Pseudomonas aeruginosa ) was(6.25 mg/ml). Conclusion: The essential oil of the Syzygium aromaticum possessed antibacterial activity against the Gram positive and Gram negative organisms which are responsible for several diseases.
Description: 67 Pages
URI: http://khartoumspace.uofk.edu/123456789/24582


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