University of Khartoum

Detection of CTX-M, TEM and SHV Genes in Gram Negative Bacteria Isolated From Nosocomial Patients at Port Sudan Teaching Hospital

Detection of CTX-M, TEM and SHV Genes in Gram Negative Bacteria Isolated From Nosocomial Patients at Port Sudan Teaching Hospital

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Title: Detection of CTX-M, TEM and SHV Genes in Gram Negative Bacteria Isolated From Nosocomial Patients at Port Sudan Teaching Hospital
Author: Osman, Abdelrahman Mustafa Abdelrahman
Abstract: Background Bacteria from clinical and non-clinical settings are becoming increasingly resistant to conventional antibiotics and broader information-control problem. Patients admitted to hospitals for the treatment of resistant bacterial infections are adding to the already too high costs of healthcare and are a source of resistant bacteria and/or resistance-encoding genes. Methodology This was a descriptive, cross-sectional study, conducted from 2011 to 2014 among hospitalized patients suffering from Gram negative bacilli infection. Four hundred specimens were collected. Isolation and identification of pathogenic bacteria were carried out following standard laboratory procedures including API 20 E identification system. All isolates were tested to 14 types of commonly antimicrobial uses. Identification of ESBLs production was performed by the double disk synergy test and double disk diffusion test. ESBLs positive specimens were tested for the presence of ESBLs encoding genes using PCR with specific primers for the detection of CTX-M, TEM and SHV genes. Results were analyzed using (SPSS). Results One hundred ninety eight types of pathogenic Gram negative bacilli were isolated. The major isolates were Escherichia coli (44.9%), followed by Klebsiella pneumoniae (23.2%) and the least Gram negative bacilli isolated were Klebsiella oxytoca, Serratia marcescens, Morganella morganii, Salmonella paratyphi A and Citrobacter koseri (0.5%). ESBLs producing bacteria were Escherichia coli (63.6%). The result of the antimicrobial susceptibility testing showed that amikacin was most effective drug (96.6%) followed by chloramphenicol (63.6%). While the maximum resistance rate (100%) was detected against ceftriaxone, ciprofloxacin and tetracycline. The presence of ESBL genes was detected in Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Serratia odotifera and Enterobacter sakasaki. Conclusion The frequency rate of ESBL-producing Gram-negative bacilli in the population of Red Sea State is higher than that prevalent in other countries. The commonest ESBL gene in this population was CTX-M gene followed by TEM gene and SHV gene. The double-disc synergy and double disk diffusion tests are reliable for detection of bacterial ESBL production.
URI: http://khartoumspace.uofk.edu/123456789/25019


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