University of Khartoum

Effect of Growth Regulators on In VitroMorphogenic Response of Boscia senegalensis (Pers.) Lam. Poir. UsingMature Zygotic Embryos Explants

Effect of Growth Regulators on In VitroMorphogenic Response of Boscia senegalensis (Pers.) Lam. Poir. UsingMature Zygotic Embryos Explants

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Title: Effect of Growth Regulators on In VitroMorphogenic Response of Boscia senegalensis (Pers.) Lam. Poir. UsingMature Zygotic Embryos Explants
Author: Daffalla, Hussien H.; Abdellatef, Eltayb; Elhadi, Elsadig A.; Khalafalla, Mutasim M.
Abstract: The percent study describes the in vitro responses of mature zygotic embryos of Boscia senegalensis to different concentrations (0.0–5.0 mg/L) of 6-benzyladnine (BA), Thidiazuron (TDZ), α-Naphthalene acetic acid (NAA), and 2, 4-Dichlorophenoxyacetic acid (2, 4-D) supplemented on Murashige and Skoog medium (MS). The plant growth regulators (PGRs) were considerably affected the morphogenetic responses. BA produced adventitious shoots through two ways: direct organogenesis and auxiliary shoot formation. Both 2, 4-D and TDZ tend to produce callus, whereas NAA improve the development of embryos to seedlings. Maximumnumber of shoots/explant (14.8 ± 0.6) was obtained onMSmediumsupplemented with 3.0 mg/L BA. 67.0% of excised shoots were rooted either on 1/2MS medium augmented with or without 0.25mg/L IBA. The highest number of roots (1.2 ± 0.4) and root length (0.5 ± 0.2 cm) was produced on 0.25mg/L IBA-containing medium. Regenerated plants were successfully acclimatized and transferred to the green house with 70% survival rate. All the plants appeared morphologically uniform with normal growth pattern. A rapid (30 days), efficient and without subculturing protocol for in vitro regeneration of B. senegalensis was developed.
URI: http://khartoumspace.uofk.edu/123456789/25156
Date: 2011


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