University of Khartoum

Detection of Oxytetracycline Residues in Table Eggs in Khartoum State

Detection of Oxytetracycline Residues in Table Eggs in Khartoum State

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Title: Detection of Oxytetracycline Residues in Table Eggs in Khartoum State
Author: Abdelgadir, Hind Eltahir Ahmed
Abstract: This study was conducted to detect the presence of oxytetracycline residues in table eggs in Khartoum State and to compare the level of oxytetracycline residues in table eggs with internationally acceptable maximum residue limits (MRLs). One hundred and eighty table egg samples were randomly collected from 18 sale points in three localities in Khartoum State, (60 eggs from Omdurman, 60 eggs from Khartoum and 60 eggs from Khartoum North) during August and September, 2015. Microbiological inhibition assay was used to screen the presence of antibiotic residues using Bacillus subtilis seeded in nutrient agar. Ninety positive egg samples resulted from microbiological inhibition assay were analyzed to detect the presence and quantify oxytetracycline residues by using HPLC.The results of microbiological inhibition assay showed that 50% of the collected samples were positive for antibiotic residues in 16 sale points in Omdurman, Khartoum and Khartoum North with percentages 18.9%, 15.6%, and 15.6%, respectively. HPLC results showed that 70% were positive for oxytetracycline residues above the permissible maximum residue limits in16 sale points in the three localities, Omdurman, Khartoum, Khartoum North and Omdurman with percentages 21.2%, 23.3%, 25.5%, respectively. It was concluded that high percentage of table eggs contained oxytetracycline residues above the maximum residue limits that indicated the widespread misuse of oxytetracycline in poultry farms that may cause health hazards to the consumer in Khartoum State. Therefore the study recommends compliance of drug withdrawal periods in poultry farms can reduce the incidence of antibiotic residues in order to protect consumer health.
Description: 58 Pages
URI: http://khartoumspace.uofk.edu/123456789/25214


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