University of Khartoum

Antimicrobial Resistance and Molecular Typing of Campylobacter spp. Isolated from Poultry in Khartoum State

Antimicrobial Resistance and Molecular Typing of Campylobacter spp. Isolated from Poultry in Khartoum State

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Title: Antimicrobial Resistance and Molecular Typing of Campylobacter spp. Isolated from Poultry in Khartoum State
Author: Elsafi, Hassan Elsiddig Hassan Hag
Abstract: Campylobacter spp. are reported as the most common cause of bacterial gastroenteritis in the world. This study was conducted to determine the antimicrobial resistance and genetic diversity of Campylobacter spp. in poultry in Khartoum State. In this study a total of 37 Campylobacter isolates were obtained from 679 fecal samples from 8 poultry farms in Khartoum State. The isolates were identified to the species level using multiplex polymerase chain reaction method which revealed C. coli (30 isolates) and C. jejuni (7 isolates). Antimicrobial susceptibility testing was determined by using minimum inhibitory concentration method (MIC), indicated that all isolates were sensitive to erythromycin. The 37 isolates were found to be resistant to tetracycline (91.9%), ciprofloxacin and nalidixic acid (81.1%), gentamycin (45.9%) and streptomycin (5.4%). Sixteen isolates (43.2%) demonstrated multiple drug resistance. The tet(O) gene was detected in all the isolates which demonstrated resistance to tetracycline. All isolates were typeable by flaA molecular typing method. The flaA molecular typing demonstrated 16 different flaA types among the 37 isolates which indicates great diversity within the 37 Campylobacter isolates. This study demonstrated that poultry farms in Khartoum State were infected by Campylobacter spp. characterized with high and multiple resistance to some antimicrobial agents. Further studies are necessary to determine the prevalence of Campylobacter in poultry and other animals.
Description: 131 Pages
URI: http://khartoumspace.uofk.edu/123456789/25332


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