University of Khartoum

In-Vitro screening for Anti-Oxidant and Anti-Breast cancer activities of some medicinal plants

In-Vitro screening for Anti-Oxidant and Anti-Breast cancer activities of some medicinal plants

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Title: In-Vitro screening for Anti-Oxidant and Anti-Breast cancer activities of some medicinal plants
Author: Taha, Mazen Mohamed Mustafa
Abstract: Background: The use of medicinal plants for healing of various human diseases backdated to ancient times. Therefore, study and scientific validation of the traditional use of medicinal plants is a possible mean towards development of economical and effective drugs from indigenously available medicinal plants. With more than 100 types, cancer remains the third leading cause of death worldwide following cardiovascular and infectious diseases with breast cancer being the most common form of cancer among women in Sudan. Oxidative stress has been repeatedly linked to many of today's diseases such as cancer. The current study is aimed to evaluate antioxidant, anti-breast cancer activity as well as phytochemical constituents of fourteen medicinal plants, those were: Lawsoniainermis (leaves), Capparis decidua (arial parts), Hydnora abyssinica (rhizome) ,Cymbopogon citratus (arial parts), Grewiavillosa (leaves),Kigelia africana (fruits), Brassica oralceae (leaves), Trigonellafoenum (seeds), Lantana camara (roses), Aristolochia bracteolata (roots),Nigella sativa (seeds), Ricinuscommunis (leaves), Moringaoleifera (roots), and Chamomilla recutita (flowers). Material and Methods: Plants were collected from different parts of Sudan and authenticated at the herbarium of the Aromatic and Medicinal Plants Research institute. The samples were air-dried for twoweeks and powdered. A hundred gram of each plant powder was extracted exhaustively with absolute ethanol (97%, 200ml) using Soxhlet extractor for 3 hours. The extracts were filtered andconcentrated on a rotary evaporator at 45ºC and reduced pressure and kept for further investigations. The extracts were subjected to qualitative phytochemicalanalysis for various constituents. Anti-oxidant activity of the investigated plants was evaluated by diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and iron chelating activity assay methods. The cytotoxicity of each extract was assessed against human breast cancer (MCF7) cell line. Results: Phytochemicalanalysis of the plant extracts investigated revealed the presence of considerable amount of alkaloids, flavonoids, sterols and carbohydrates in all plant ethanol extracts under study, whereas, saponins were only detected in ethanol extracts of Trigonellafoenum-graecum (seeds),Aristolochia bracteolata (roots),andNigella sativa (seeds). On the other hand, cardiac glycosides were detectedinCapparis decidua (arial parts) and Kigelia africana (fruits), whilecoumarins were detected only inKigelia africana (fruits) and anthracenes were not detected in any of the ethanol extracts under study. Regarding anti-oxidant activity, plant extracts from L. inermis, L. camara H. abyssinica, G. villosa, andR. communis, ranked as the top five most active plant extracts among the 14, exhibited strong activityon scavenging DPPH radicals with the determinedradical scavenging activity (% RSA) values 93± 0.01, 92±0.01, 74±0.14, 61±0.04, and 45±0.02 respectively. On the other hand, Plant extracts from T. foenum-graecum, C. decidua, B. oralceae, A. bracteolata , andR. communis, ranked as the top five most active plant extracts among the 14, showed strong activityon iron chelating ability with the determined % iron chelating ability values 68±0.03, 50±0.01, 46±0.10, 44±0.01 and 38±0.02 respectively. With respect to anti-breast cancer activity, all extracts from the plants studied showed marked cytotoxicity against MCF7 cells after incubation for 48 hrs, with L. inermis andA.bracteolata being the most potent with IC50 of 19.1µg/ml. The established anticancer drug, doxorubicin, was included into the cytotoxicity testing as a reference control drug and the reported IC50was 4.20 µg/ml. Conclusion: The results of the current study provided information related to the possible use of some Sudanese medicinal plants, and mostly L. inermis and A. bracteolata as alternative novel nutraceutical products in the treatment of breast cancer. Furthermore, these findings helped to verify the claimed traditional use of these plants.
URI: http://khartoumspace.uofk.edu/123456789/25356


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