University of Khartoum

Development of new spectrophotometric methods for the determination of Sulfadoxine and Pyrimethamine in bulk and pharmaceutical formulations

Development of new spectrophotometric methods for the determination of Sulfadoxine and Pyrimethamine in bulk and pharmaceutical formulations

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Title: Development of new spectrophotometric methods for the determination of Sulfadoxine and Pyrimethamine in bulk and pharmaceutical formulations
Author: Osman, Myada Hashim Mohamed
Abstract: SUL and PYR formulation is widely used in many endemic areas. These drugs are available in combination of 20: 1. Such combination at this ratio is a challenge to the pharmaceutical analyst. HPLC which is an official method is definitely a solution to this problem; however development of a feasible and cost effective spectrophotometric method for the determination of each component in the mixture would be of more benefit especially in development countries. Therefore the objective of this work was to develop and validate simple spectrophotometric methods for determination of sulfadoxine and pyrimethamine in pure bulk form and pharmaceutical formulations. Methodology: Different spectrophotometric methods were developed for the determination of SUL and PYR in bulk and dosage forms. Method I was based on dissolving SUL in 1M NaOH without interference with PYR which is insoluble in 1M NaOH (direct assay without extraction method). The absorbance of the dissolved SUL in 1M NaOH was measured using UV spectrophotometer. Method II was based on their separation using 1M NaOH in which SUL is soluble and CHCl3 in which PYR is soluble. The absorbance of each separated constituent was then measured using UV spectrophotometer. Method III was based on the fact that diluting a solution of both constituents in CH3OH cancels the absorbance of PYR, which is present in very low ratio (swamping method). The obtained absorbance of this solution at wavelength 274nm will be only for SUL without interference with PYR. The developed methods were statistically validated at 95% confidence limits. Results: The developed methods were found to obey Beer,s law over the concentration range 2- 10µg/ml with correlation coefficient not less than 0.999. The sensitivity of the methods was proved by the obtained low values of LOD and LOQ ranging between 0.213-0.292 µg/ml and 0.534-0.885 µg/ml, respectively. Moreover, RSD% values were found to be less than 2%, which reflected the precision of the developed methods. Conclusion: Simple, accurate, precise and sensitive spectrophotometric methods have been developed for the simultaneous determination of SUL and PYR in combination and for their determination after being separated in bulk and pharmaceutical dosage forms.
URI: http://khartoumspace.uofk.edu/123456789/25358


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