University of Khartoum

Association between Helicobacter pylori and some other factors with oesophageal and gastric cancer in Sudanese patients

Association between Helicobacter pylori and some other factors with oesophageal and gastric cancer in Sudanese patients

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Title: Association between Helicobacter pylori and some other factors with oesophageal and gastric cancer in Sudanese patients
Author: Mohammed, Rihab Ahmed Hassan
Abstract: Background: Helicobacter pylori (H. pylori) infection is a major risk factor for developing gastric cancer. Epidemiologic evidence accumulating suggests that H. pylori infection, especially with strains carrying the cytotoxin associated gene A (CagA), is associated with the risk of adenocarcinoma of the esophagus or gastro- oesophageal junction by stimulating the production of nitrosamines. The study aimed to estimate the association of H. pylori in Sudanese patients with gastric and oesophageal cancer, detect the presence of IgG and IgA antibodies to different H. pylori antigens and investigate the association between H. pylori infection and age, gender, water sources, residence, cigarette smoking and alcohol consumption. Materials and Methods: A total of 124 subjects were included in this study: 18 were histopathlogically confirmed gastric cancer patients, 44 were oesophageal cancer patients (30 with Stratified squmaous cell carcinoma ((SCC)) and 14 with adenocarcinoma of oesophagus) and were considered as case group; 62 were control subjects who matched the patients with respect to age and sex. Serum samples (5ml) were collected from each patient and healthy persons, and analyzed for H. pylori IgG and IgA antibodies against H. pylori antigens (120 kda and 95,75,67,66, 57, 54, 50, 41, 33, 30, 29, 26, 19 and 17 kda) using Western Immunoblotting. According to kits guidelines of Euroimmune catalogue, H. pylori was considered positive when at least one or two distinctive combined antigen bands or minimum of two weak antigen band (combination antigens with the molecular weights 17 kDa, 19 kDa, 26 kDa, 30 kDa, 33 kDa, 95 kDa, and 120 kda) and it was considered negative when no antigen band or antigen bands were present. Results: H. pylori combination antigens were present in 59 (95.2%) of case group and 59 (95.2%) of control subjects with no significant difference (P= 0.66). While, all case groups 62(100%) were positive for H. pylori antigen Cag 120 kda, only 59 (95.2%) of control group were positive (P = 0.122). Patients with oesophageal adenocarcinoma had a somewhat lower overall prevalence of H. pylori antibodies than control subjects (85.7% versus 95.2%), but a somewhat similar prevalence of 120 kda antibodies (100% versus 95.2%). However, antibodies to H. pylori Vac antigen (95 kda) were present in (65.4%) (n=40) of case group, (83.3%) (n=25) of SCC and none of adenocarcinoma and (83.3%) (n=15) of gastric cancer patients and (17.7%) (n=11) of control subjects, and with significant difference (P <0.0001). H. pylori was not associated with age, gender, water sources, residence, cigarette smoking and alcohol consumption. Conclusions: The present study showed that the prevalence of H. pylori antibodies were the same in the case group and the control group. H. pylori Cag antigen 120 kda is common in case and control groups. However, H. pylori Vac antigen 95kda is more specific in diagnosing patients with oesophageal and gastric cancer; hence all H. pylori positive cases should be tested serologically to detect which antigen is present to avoid cancer development. Male and female have the same risk of H. pylori infection, and at any age can be infected with H. pylori.
URI: http://khartoumspace.uofk.edu/123456789/25367


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