University of Khartoum

Bio typing and Molecular Characterization of Bacteria Isolated from Touch Screen Mobile among Health Workers in some Hospitals in Khartoum.

Bio typing and Molecular Characterization of Bacteria Isolated from Touch Screen Mobile among Health Workers in some Hospitals in Khartoum.

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Title: Bio typing and Molecular Characterization of Bacteria Isolated from Touch Screen Mobile among Health Workers in some Hospitals in Khartoum.
Author: Elginawi, Eiman Elmazri Galal Eldien
Abstract: Background: Touch screen mobile phones (TSMPs) have become an indispensable part of our lives. Offering plethora of advantages, however they are prolific breeding grounds for pathogens in health care workers (HCWs). In Sudan there is increase in the use of mobile phones in the last ten years among the general population. The use of phones is common in certain areas of the environment where the presence of bacteria is high like hospitals. This study aimed to determine the potential hazards of mobile phones to harbour microorganisms in hospital environments, moreover to compare the results of culturing and molecular testing by using ribosomal RNA gene (16S rRNA) sequence analysis for identification and classification of bacteria. Material and Methods: A cross-sectional study was conducted from October, 2014 to January, 2015. One hundred and fifty swab samples were randomly collected from health care workers from various departments in three different Hospitals in Khartoum State namely Radiation and Isotopes Centre Khartoum), Khartoum Teaching Hospital and Elshab Teaching Hospital . Fifty samples were selected from each hospital including: 35 medical laboratory technologists, 32 nurses, 28 doctors, 18 nutrition and immunization workers , 12 pharmacists, 9 cleaners, 6 statistics workers , 5 operation room staff and 5 X- Ray staff . Samples were cultured and processed via standard microbiological procedures and further identified by both conventional and molecular techniques using 16S rRNA sequence analysis. Result: A total of 131 isolates were obtained from 150 swabs of TSMPs with a total percentage of 87.3%.There was one type of bacteria in 120 samples, two types in 11 and 19 samples showed no growth. The highest viable counts were observed among TSMPs owned by medical laboratory technologists while the least viable count was on those owned by nurses. All samples obtained from doctors, operation room staff, pharmacists, and statistics workers showed growth(100%), followed by nutrition and immunization workers by (94.4%). Contamination in the nursing staff was (81.2%), in X- Ray staff and laboratory technologists was (80%) and (77.1%) respectively. The least contamination ratio was among health cleaners (66.7%). The most common organisms isolated were non-spore forming Gram Positive Bacilli, B. cereus , S. aureus , Coagulase Negative Staphylococci , Enterococci, E.coli , P. aeruginosa , K. Pneumoniae , P. maribilis , and S.enterica . A comparative analysis was made between the conventional method and 16S rRNA gene sequencing at genus and species levels. The BLAST searching and BioEdit multiple sequence alignment revealed mutation for one isolate at highly conserved region which was reported as new strain of (Salmonella. enterica strain 1S Gene Bank KX 443366). Conclusion: TSMPs represent a major source of infection by potentially pathogenic bacteria which might be transmitted from health care workers to patients and public. The use of 16S rRNA gene sequencing in diagnosis and identification of pathogenic bacteria is reliable, accurate and specific method.
Description: 96 Pages
URI: http://khartoumspace.uofk.edu/123456789/25413


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