University of Khartoum

Chemotaxonomy of Selected Members of the Plant Family Zygophyllaceae in Sudan

Chemotaxonomy of Selected Members of the Plant Family Zygophyllaceae in Sudan

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Title: Chemotaxonomy of Selected Members of the Plant Family Zygophyllaceae in Sudan
Author: Rahamtalla, Hind Ahmed Babikir
Abstract: The objectives of this study include what was accomplished in revising the reclassification of Desert date (Balanitis aegyptiaca) and its affiliation to the Zygophyllaceae family with the Caltrop (Tribulus) group, Cretan prickly clover (Fagonia indica) and Caper beans (Tetrarna) group. Different analytical methods and morphological characteristic and the analysis of amino acids by ninhydrin, thin layer chromatography and amino acid analyzer were applied. While the proteins were analyzed by Biuret method and gel electrophoresis. General phytochemical screening was used and Gas chromatography/ Mass spectrophotometry (GC/MS) technique was used to compare the secondary metabolites of the selective plants. To estimate bioactivities, the Brine shrimp method was used for determination of cytotoxicity, whereas compound 2,2-diphenyl-1-picryl hydrazyl method (DPPH) was used to study the antioxidant activities. Also, the deoxyribonucleic acids were analyzed by using random amplified polymorphic DNA (RAPD). Statistical analyses were carried out for the obtained results. Morphological analysis supported the suggested classification by angiosperm phylogeny group (APG) 2016. The amino acids results differed according to the applied methods; for example the ninhydrin method ended up into two groups: the first one composed of two species, Tribulus terrestris and T. pentandrus, while the other was formed from the rest of the plants. Thin layer chromatography method divided the plant species into two groups in line with the new suggested classification; except for F. indica which can fit in both groups but with different degrees. However, using the amino acid analyzer showed substantial degrees of similarities among the different plants. Analysis of proteins by using Biuret method divided the plants into three groups: the XVI first group is represented by F. indica while the rest of plants except Tetraena simplex and T. pentandrus represented the third group. Estimation of proteins by electrophoretic method showed agreement with new classification system. The result of the chemical screening proved the presence of all investigated compounds in all selective plants. While the mean paired affinity values for the retention factor (Rfs) showed close relationships among members of the Tribulus spp. and B. aegyptiaca and also between F. indica and Tetraena alba and hence, these results are in agreement with the newly revised classification (2016). Cytotoxicity study confirms the presence of high toxicity in the case of F. indica which did not appear in the other plants. While the antioxidant activity studies revealed the characterization of sub-family Zygophylloidea by the highest activities. This result is in agreement with the suggested classification into two subfamilies. The use of GC/M S lead to the appearance of different- relationships among the studied plants, with the presence of very close relationships between Tribulus spp. and B. aegyptiaca which support their belonging to the same subfamily. On the other hand, weak relationship was observed in the case of B. aegyptiaca compared to F. indica and T. alba which is contrary to its relationship with Tetraena simplex that indicated strong relationship with all the members of the two sub-families. The DNA results revealed close relationship between Tetraena spp., F. indica, Tribulus spp. and B. aegyptiaca which showed its belonging to the sub- family Zygophylloideae. Generally the obtained results pointed to additional proofs which assure the soundness of the revised suggestion which appeared in the classification system angiosperm phylogeny group for the year 2016.
URI: http://khartoumspace.uofk.edu/123456789/25605


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