University of Khartoum

Evaluation of Bacterial Contamination of Bovine Carcasses at Elsabloga Slaughterhouse, Khartoum State, Sudan

Evaluation of Bacterial Contamination of Bovine Carcasses at Elsabloga Slaughterhouse, Khartoum State, Sudan

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Title: Evaluation of Bacterial Contamination of Bovine Carcasses at Elsabloga Slaughterhouse, Khartoum State, Sudan
Author: Mousa, Nazik Adam Yagoub
Abstract: The present study was conducted at Elsabloga slaughterhouse, Khartoum State-Sudan during January - March 2016. To evaluate the bacterial load and level, isolation and identification of bacterial types on bovine carcasses. A total of 90 swabs were collected from shoulder, flank and rump (30 for each) of bovine carcasses after last washing. The samples were inoculated in peptone water and viable count of bacteria was carried out, using peptone water as diluents to make tenfold dilution from each sample. Then, 10-4, 10-5 and 10-6 dilution were cultured in duplicates onto nutrient agar. The peptone water culture was sub cultured onto blood agar and inoculated in selenite broth, followed by sub-culturing onto MacConkey's agar plates. The data were subjected to analysis using SPSS; the bacterial counts were converted to log10 and t-test was performed. The results revealed that the mean viable count of bacteria were 7.79 ±1.26 colony forming unit per centimeter square in the shoulder, 7.92 ±1.08 cfu /cm2 in the flank and 7.69 ±1.54 cfu/cm2 in the rump. There were no significant differences at P < 0.05. A total of 288 isolates were obtained; 103 (35.76%) from the shoulder, 96 (33.33%) from flank and 89 (30.90%) from the rump. The isolates were identified based on microscopic, cultural and biochemical properties. The Gram-positive bacteria were Bacillus spp. 60 (20.83%), Micrococcus spp. 50 (17.36%), Staphylococcus aureus 31(10.76%), Staphylococcus epidermidis 13(4.51%), Staphylococcus xylosus 12(4.16%), Corynebacterium spp. 11(3.82%), Propionibacterium spp. 10 (3.47%), Staphylococcus sciuri 7(2.43%), Actinomyces spp. 6(2.08%), Staphylococcus equorum 5(1.74%), Nocardia spp. 3(1.04%), Staphylococcus lentus 2(0.7%), while Gram-negative bacteria were Escherichia coli 35(12.15%), Proteus penneri 18(6.25%), Pseudomonas aeruginosa 5(1.74%), Streptobacillus spp. 5(1.74%), Klebsiella pneumoniae 5 (1.74%), Shigella spp. 3(1.04%), Citrobactor koseri 3(1.04%), Proteus mirabilis 2(0.7%), Salmonella spp. 2(0.7%). Gram-positive bacteria species prevailed in the shoulder, flank and rump, out of 21 bacterial species, 12 species were Gram-positive and 9 species were Gram-negative. It is concluded that the level of bacterial contamination in bovine carcasses at Elsabloga slaughterhouse was very high and constituted a real public health hazard as pathogenic and toxinogenic. It is recommended that proper washing of bovine carcasses using treated water should be applied during slaughtering processes to reduce the level of contamination with microorganisms.
Description: 65 page
URI: http://khartoumspace.uofk.edu/123456789/26058


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