University of Khartoum

Effects of Ambrosia maritima (Damsissa) on Anaemia Related Parameters in Rabbits

Effects of Ambrosia maritima (Damsissa) on Anaemia Related Parameters in Rabbits

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Title: Effects of Ambrosia maritima (Damsissa) on Anaemia Related Parameters in Rabbits
Author: Alhassan, Marwa Hassan Alfadol
Abstract: : The study was carried out to investigate the anti- anaemic activity of traditional medicinal plant, Ambrosia maritima (Damsissa) ethanolic extract in phenylhydrazine hydrochloride-induced anaemia in rabbits. Twenty five adult rabbits were equally divided into 5 groups. Anaemia was induced by subcutaneous administration of phenylhydrazine hydrochloride at a dose of 30 mg/kg body weight and maintenance dose of 15 mg/kg body weight in all groups except group (A) (control group).On day 8 of administration of phenylhydrazine, the ethanolic extract of Ambrosia maritima was orally administered to groups B, C, D at the rate of 250, 500, 1000 mg/kg body weight respectively, meanwhile group E was left as phenylhydrazine control group (phenylhydrazine without Ambrosia maritima). The experiment continued for 30 days during which, blood samples were collected on day 0, 7, 14, 22, 30 for heamatological examinations and serum analysis. The haematological parameters investegated included total erythrocytes count (TEC), Packed cell volume (PCV), Mean corpuscular volume (MCV), Mean corpuscular haemoglobin (MCH), Mean corpuscular haemoglobin concentration (MCHC), total leukocytes count TLC and differential leukocyte count. Serum biochemical tests included Fe concentration, total lipid, total protein, cholesterol and blood glucose level. The results of the haematological tests revealed significant (P<0.05) increase in Hb, TEC, PCV, TLC and the ratios of lymphocytes, monocyte and basophilsand significant (P<0.05) decrease in MCV, MCH, eosinophils and neutrophil in response to Ambrosia maritima ethanolic extracts.No significant changes were observed in MCHC and blood glucose level. The results of serum metabolites revealed significant (P<0.05) increase in total lipid and Fe concentration and significant (P<0.05) decrease in cholesterol level. No significant changes were observed in Total protein values in response to Ambrosia maritima treatment.Ambrosia maritima ethanolic extract orally had no toxic effect at the different doses used in this study (250,500 and 1000 mg/kg body weight).The data demonstrated that Ambrosia maritima had anti-aneamic effect. Further Studies are needed to investigate the effect of Ambrosia maritima by other methods of extraction at higher doses and for a longer period of time using different routes of administration
URI: http://khartoumspace.uofk.edu/123456789/26361


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