University of Khartoum

Effect of Electric Field on Growth and Development of Five Guar (CyamopsistetragonolobaL.) Genotypes

Effect of Electric Field on Growth and Development of Five Guar (CyamopsistetragonolobaL.) Genotypes

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Title: Effect of Electric Field on Growth and Development of Five Guar (CyamopsistetragonolobaL.) Genotypes
Author: Sharaf Eldin, Bara’ah Ali Eltahir
Abstract: The study aimed to investigate the effect of the application of external electric field on the growth and development of five genotypes of Cyamopsis tetragonoloba L. plant (Gm2, Gm5, Gm6, Gm8 and Gm34). This effect is expressed in terms of electric energy density created within pairs of copper plates forming electric capacitors in a DC current circuit connected to an electric source. Two systems were designed and used for experimentation. The first one with two copper plate's area (25 and 225) cm2 and different voltage levels (0, 6, 9, 22.1 and 30) volts and the second one with 1500 cm2 plate's area connected with six volts. Seeds of C. tetragonoloba were located within spaces between each capacitor’s plates and are exerted upon by different energy densities. The obtained data were statistically analyzed. The results showed that when five treatments (0, 6, 9, 22.1 and 30 volts) were used to five genotypes in 12 days after treatments; all treatments resulted in inhibition of root growth and plant height in the germinating seeds of five genotypes either when 25 cm2 or 225 cm2 plate's area were used. Also, when 25 cm2 and 225 cm2 plate's area were used with one treatment (6 volts) for the five genotypes; 225 cm2 plates were found more affective in the reduction of plant height than 25 cm2 plates. When 25 cm2 plates were used the highest reduction of radicle length and plant height was obtained by 9 volts. When the five genotypes were exposed to 6 volts by using 225 cm2 plates; the genotype that was affected by the treatment in reducing radicle length and plant height was Gm2 in 3rd and 4th day after treatment. The less affected one was Gm5. When treating genotype Gm34 with 6 volts in different plant growth stages during a period 8 weeks; the highest percentage in plant height, number of leaves, number of branches and stem diameter was obtained when treating plant in vegetative stage. All treatments accelerated flowering and fruiting date. The effect of electric field treatment was not detected when root tip tissue sections were examined. When radicle, hypocotyl and cotyledonary leaf explants of five genotypes of C. tetragonoloba: Gm2, Gm5, Gm6, Gm8 and Gm34 were cultured in Murashige and Skoog media supplemented with different growth regulators, results show that M2 (with NAA) failed to form callus for the five genotypes, while M1 (with IBA) and M3 (with 2-4, D) initiated callus for the five genotypes. M3 was better than M1 for callus formation for the C. tetragonoloba genotypes. It was concluded that the five genotypes of C. tetragonoloba have responded differently to the exposure of electric field doses at different stages of development.
URI: http://khartoumspace.uofk.edu/123456789/26478


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