University of Khartoum

Morphological and Molecular Characterization of some Cowpea (Vigna unguiculata L.) Genotypes collected from Sudan

Morphological and Molecular Characterization of some Cowpea (Vigna unguiculata L.) Genotypes collected from Sudan

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dc.contributor.advisor Seif Eldin Mudawi Gasim en_US
dc.contributor.author Khalafalla, Ahmed Abdallah Eltayeb
dc.contributor.other Agronomy en_US
dc.date 2017-01
dc.date.accessioned 2018-07-11T12:39:46Z
dc.date.available 2018-07-11T12:39:46Z
dc.date.submitted 2018
dc.identifier.uri http://khartoumspace.uofk.edu/123456789/26600
dc.description 90page
dc.description.abstract The aim of this study was to characterize some cowpea genotypes collected from Sudan for qualitative and quantitative traits and to assess the genetic diversity among the accessions based on Random amplified polymorphism DNA (RAPD). Therefore, a field experiment was conducted at the Demonstration Farm of the Faculty of Agriculture, University of Khartoum for one season (2014/2015) to characterize 14 Sudanese cowpea genotypes for qualitative and quantitative traits based on the International Board for Plant Genetic Resources (IBPGR) for cowpea descriptors. A randomized complete block design with three replications was used for laying out the field experiment. Data were collected and subjected to analysis of variance, genotypic coefficient of variability, heritability, genetic advance and interrelationships among the studied traits. Moreover, cluster analysis using twenty RAPD markers was carried out to study the genetic similarity among the fourteen genotypes. The results of the analysis of variance showed significant differences among the 14 genotypes for most of the studied traits, except leaf to stem ratio, stipule length and stipule width. Most of the studied traits recorded greater genotypic variance than environmental variances, except yield (Kg/ha), leaf to stem ratio, number of locules/pod, pod width and stipules width. The highest genotypic coefficient of variation (53.5%) and genetic advance as percentage of the mean (88.7%) were recorded for number of pods per plant. Other traits that showed high genotypic coefficient of variation were yield per plant (49.5%), number of racemes per plant (42.8%) and yield per hectare (41.7%). High heritability (above 80%) was obtained for days to 50% pods maturity (86%) and terminal leaflet width (81%). Low estimates (below 50%) were found for number of locules per pod (41%), seed per plant (40%), pod width (33%), stipule length (30%), stipule width (25%) and leaf stem ratio(24%). Yield per hectare exhibited highly significant positive genotypic association with number of racemes per plant (97%), number of pods per plant (91%), yield per plant (88%), peduncle length (82%) and number of pods per peduncle IX (71%). Seed yield per plant showed highly significant positive genotypic and phenotypic association with number of racemes per plant, number of pods per peduncle and number of pod per plant. The association of yield per plant and per hectare with number of days to 50% flowering and days to 50% mature pod was not significant. On the other hand, the association between the number of branches on main stem with peduncle length and number of racemes per plant and number of pods per plant were positive and significant at genotypic and phenotypic levels. The number of locules/pod gave highly significant negative genotypic correlation with peduncle length and significant negative correlation with racemes/plant. Among the 20 RAPD markers that used to characterize the genetic diversity of the 14 genotypes, 379 amplified bands were generated by 18 primers with polymorphic information content ranging from 0.63 to 0.98 with an average of 0.9. The number of fragments detected ranged from 1 for OPL-11 to 51 for OPY-2. The results showed the presence of one to five (1-5) unique fragments of different sizes for particular genotypes, HYDOOB and HSD-5674 (5fragments), HSD-5670 (3fragments), HSD-5131 (2fragments) and HSD-2967, HSD-5671, HSD-5672, HSD-5130 and HSD-5864 (1fragments). Genetic similarity among the genotypes based on the RAPD markers ranged from 0.02 to 0.47 with an average of 0.25. Highest genetic similarity index (0.47) was between genotypes HSD-2966 and HSD-2967 and between genotypes HSD-5131and HSD-5627, whiles the lowest genetic similarity was shown between the genotypes HSD- 5131 and HYDOOB followed by that between HSD-2976 and HSD-29130 genotypes. The present study indicates that the large variation among the genotypes used, for the studied characters, it provides a wide scope for improvement through selection. Moreover, molecular markers could be used to obtain fast, accurate and high throughput fingerprinting that revealed the existence of significant variation among the 14 genotypes which could be exploited to produce improved genotypes. en_US
dc.language.iso en en_US
dc.publisher University of Khartoum en_US
dc.subject Morphological en_US
dc.subject Molecular Characterization en_US
dc.subject Cowpea (Vigna unguiculata L.) en_US
dc.subject Genotypes collected en_US
dc.subject Sudan en_US
dc.title Morphological and Molecular Characterization of some Cowpea (Vigna unguiculata L.) Genotypes collected from Sudan en_US
dc.type Thesis en_US
dc.Degree M.Sc en_US
dc.Faculty Agriculture en_US

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