University of Khartoum

Laboratory Study on the Effect of Different Solvents on Toxcicity of Crude Ethanol Extract of Tamarix nilotica Ehrenb Leaves against Anopheles arabiensis Pattonand Culex quiquefasciatus Say Larvae

Laboratory Study on the Effect of Different Solvents on Toxcicity of Crude Ethanol Extract of Tamarix nilotica Ehrenb Leaves against Anopheles arabiensis Pattonand Culex quiquefasciatus Say Larvae

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Title: Laboratory Study on the Effect of Different Solvents on Toxcicity of Crude Ethanol Extract of Tamarix nilotica Ehrenb Leaves against Anopheles arabiensis Pattonand Culex quiquefasciatus Say Larvae
Author: Hadow, Nourelhuda Karmaldin Mohamed
Abstract: In the context of keeping future environment clean, emphasis was given to botanical pesticides and other alternatives in mosquitoes control programs. The present study is concentrated on the larvicidal activity of crude ethanol leaves extract and its fractions of Tamarix nilotica (Ehrenb): (Tamaricaceae). The late 3rd instar larvae of Anopheles arabiensis Patton and Culex quinquefasciatus Say were used for the study. Larvae of both species were subjected to different doses of different fractions of ranging from 3 to 6250 ppm following the WHO procedures. Each treatment was replicated four times. Twenty-five larvae of each mosquito strains were exposed to each concentration with a final total of 100 larvae for each concentration. Control Larvae were exposed only to water. Mortality and survival were registered after 24 and 48hrs of the exposure period. The crude ethanol extract exhibited the highest mortality rate registering a low LC50 (2 ppm for An.arabiensis and 11ppm for Cx. quinquefasciatus.), whereas the hexane fraction came at the end of the list registering a high LC50 (51 ppm for An.arabiensis and 524 ppm for Cx. quinquefasciatus ) at 24 and 48 hrs after treatment ; Significant decrease was noticed in larval population levels in the treated larvae related to untreated control. The results of phytochemical screening and chromatographic separation of the crude extract revealed the presence of tannins, flavanoides, triterpens, cardiac glycosides and absence of anthraquinones and alkaloids. These results clearly indicate the promise of the extracts of T.nilotica as mosquitocidal value, however further research is needed to critically determine the exact content of each of the above ingredients.
URI: http://khartoumspace.uofk.edu/123456789/26667


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