University of Khartoum

Immune Response to Malaria Vaccine Candidates (Glurp And Msp-3) In Individuals Naturally Exposed to Malaria In Cnteral Sudan

Immune Response to Malaria Vaccine Candidates (Glurp And Msp-3) In Individuals Naturally Exposed to Malaria In Cnteral Sudan

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Title: Immune Response to Malaria Vaccine Candidates (Glurp And Msp-3) In Individuals Naturally Exposed to Malaria In Cnteral Sudan
Author: Mohammed, Suha Aydrous
Abstract: Background: This is a descriptive cross sectional hospital based study carried out at Wad Madani pediatric and Adult University Teaching Hospital in October 2007. Malaria in this area is meso-endemic and the transmission is slightly seasonal and unstable reaching its peak during rainy season (July- October). The study aimed to measure the immune responses to malaria vaccine candidates the Plasmodium falciparum Glutamate-rich protein (GLURP) and the Merozoite surface protein 3 (MSP3) they are both targeted by human IgG and IgM antibodies. Method: A number of 90 malarial patients with Plasmodium falciparum were enrolled in the study. Enzyme linked immunosorbent assay (ELISA) was performed to detect anti- GLURP and MSP-3 IgG, IgG subclases and IgM antibodies. Blood samples were microscopically examined, 44 malaria positive samples were detected and they are due to Plasmodium falciparum, 46 samples were negative. Results: Nine (8.1%) of malaria patients have normal haemoglobin level, 61(54.9%) with mild to moderate anaemia and 20 (18%) with severe anaemia. The overall prevalence of seropositive samples among study group was moderate ranging from (53.2%, 52.2%) of IgG against R0, R2 GLURP to high (60%) against IgG1 R0, and (77.8%) against IgG3 R0. IgM had Moderate to low levels against GLURP R0, R2 (56.7%, 38.2%). The GLURP R0-R2 induced the highest levels of antibody among the same subclass while GLURP R1 induced low levels. IgG1 level against MSP3 were high (87.8%) while IgG, IgG3 had moderate levels (42.2%, 56.7%) respectively and low IgM level against MSP3 (7.8%). There was significant relationship between IgG3 against R1 GLURP and anaemia (P value 0.039). The severity of anaemia correlated to IgG3 against R1-GLURP, also there is relation between IgG3, IgM against R2 and positive blood film was found in this study (P value< 0.05). The acquisition of antibody is age dependent, there was a significant association between R0-R2 GLURP IgG level and subjects age (P value <0.05). IgG1, IgG3 level directed against GLURP R0-R2 also increased with age (P value <0.05). No significant association observed between IgM and GLURP theree conserved regions and mean of age (P value > 0.005). Otherwise no significant association observed between MSP3 IgG, IgG subclasses, IgM and mean of age (P value > 0.005). Conclusion: Natural acquired humoral responses against R0-R2 GLURP and MSP3 are prevalent among falciparum malaria patient. Further studies should be conducted to clarify whether the presence of antibody responses against GLURP and MSP3 is associated with reduction of malaria incidence. Antibody levels against the tow vaccine candidates were significant suggesting that an ideal malaria vaccine should target more than one antigen. Malaria vaccine development efforts to target different stages of the parasite life cycle, and there is growing evidence that no single antigen based vaccine is effective in controlling malaria.
URI: http://khartoumspace.uofk.edu/123456789/26671


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