University of Khartoum

Assessment of Salivary Matrix Metalloproteinase -8 Level among Cigarette Smokers with and Without Chronic Periodontitis

Assessment of Salivary Matrix Metalloproteinase -8 Level among Cigarette Smokers with and Without Chronic Periodontitis

Show full item record

Title: Assessment of Salivary Matrix Metalloproteinase -8 Level among Cigarette Smokers with and Without Chronic Periodontitis
Author: Ahmed, Hisham Kamal Mohamed
Abstract: Background Chronic periodontitis is an infectious inflammatory disease initiated and propagated by bacteria and host factors and characterized by the destruction of the tooth supporting structures. Smoking is considered as an environmental factor that act together with host cells and affect inflammatory responses to the microbial challenge. Increased salivary MMP-8 levels are associated with progressive loss of attachment in periodontitis. The present study was conducted to investigate the association of the levels of salivary MMP-8 with commonly used periodontal clinical indices of groups of chronic periodontitis patients accounting for their smoking habits. Method: A convenience sample of eighty-one subjects (53 males and 28 females) was recruited for this study. The mean age of the study population was (36.6±9.2) years. The sample population was categorized into three groups: 26 non-smoker subjects with chronic periodontitis, 26 smoker subjects with chronic periodontitis and 29 periodontally healthy subjects. The clinical periodontal parameters were assessed at six sites and it included Plaque Index(PI), Gingival Index(GI), Pocket Depth(PD) and clinical attachment loss(CAL). MMP-8 was analyzed in salivary samples using Biolegend's ELISA MAX™ standard set which is a quantitative sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The MMP-8 level expressed in nano-gram per milliliter (ng/ml). Results: The results of this study showed significant differences in clinical periodontal parameters among the three groups (PI, PD and CAL) with p values of (0.001, 0.02, 0.001 respectively). Statistical analysis using one-way ANOVA showed a significant difference between the three groups in salivary MMP-8 levels with the highest value among the smokers with chronic periodontitis. On the other hands, no significant correlations were detected between the levels of the salivary MMP-8 and the other clinical periodontal parameters (GI, PD. And CAL) among each of the study groups. Conclusion: The present study clearly shows a profound effect of smoking on salivary MMP-8 in chronic periodontitis subjects in comparison to non-smokers and periodontally healthy subjects.
URI: http://khartoumspace.uofk.edu/123456789/27066


Files in this item

Files Size Format View

This item appears in the following Collection(s)

Show full item record

Share

Search DSpace


Browse

My Account