University of Khartoum

Effect of Ethanolic Extract of Hyphaene thebaica (Doum) Fruit Pulp on Nephrotoxicity Induced by Mercuric Chloride in Rats

Effect of Ethanolic Extract of Hyphaene thebaica (Doum) Fruit Pulp on Nephrotoxicity Induced by Mercuric Chloride in Rats

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Title: Effect of Ethanolic Extract of Hyphaene thebaica (Doum) Fruit Pulp on Nephrotoxicity Induced by Mercuric Chloride in Rats
Author: Abdalla, Elham Alsayed Merghani
Abstract: This study was designed to investigate the nephroprotective effects of ethanolic extract of the Hyphaene theabica L Mart (Doum) fruit pulp against mercuric chloride induced nephrotoxicity in rats. Phytochemical screening was done for the ethanolic extract of H.theabica. Twenty four Wistar albino rats were randomly arranged into 4 groups, each of 6 rats, group 1 served as control, group 2 (treated control) was treated with a single oral dose of mercuric chloride 1mg/kg B.W/day, groups 3 and 4 were treated with mercuric chloride as in group 2, in addition they were treated simultaneously and orally with a single doses of the ethanolic extract of H.theabica at 250, 500 mg/kg B.W/day, respectively. All doses were continued for 28 days. Rats were weighed and blood samples were collected on days 0, 14 and 28 for measurement of kidney function parameters (urea and creatinine) and biochemical parameters (albumin and total protein). At the end of the experimental period rats kidneys were weighed and specimens were taken for histopathology. The results of phytochemical screening revealed presence of flavonoid, alkaloid, tannin and coumarin. The results of nephrotoxicity in mercuric chloride group (Group 2) showed significant increase (p < 0.05) in concentration of serum urea and creatinine and decrease in the total protein and albumin compared to the control group. The result of nephroprotective groups (groups 3 and 4), revealed significant(p < 0.05) improvement of the previous parameters indicated by, decrease in the serum urea and creatinine and significant(p < 0.05) increase in total protein and albumin compared to the treated control (group2).The histopathological results of the kidney sections of mercuric chloride group demonstrated glomerular and tubular necrosis with desquamation of the tubular epithelial cells of the renal cortex while the groups treated with H.thebaica revealed significant amelioration of kidney damage evidenced by mild congestion and mild fatty changes of the renal tubules. The heamatological picture showed significant (p < 0.05) decrease in hemoglobin concentration and MCHC in group 2, the same parameters were significantly (p < 0.05) increased in H.thebaica treated groups while no significant changes were detected in RBCs, PCV and MCV. Body weight gain and kidney weights were significantly decreased in the intoxicated group while they were significantly improved (p < 0.05) in the treated groups. In conclusion, the ethanolic extract of H.thebaica fruits pulp at doses 250, 500 mg/kg B.W on nephrotoxicity induced by mercuric chloride in rats, exhibited significant nephroprotective effects. The recommendation of this study, focused on isolation and identification of the active components in H.thebaica that were responsible for nephroprotective activity.
URI: http://khartoumspace.uofk.edu/123456789/27103


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