University of Khartoum

Association of Il-16 Gene Polymorphism with Ischemic Stroke in Sudanese Patients

Association of Il-16 Gene Polymorphism with Ischemic Stroke in Sudanese Patients

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Title: Association of Il-16 Gene Polymorphism with Ischemic Stroke in Sudanese Patients
Author: Ahmed, Haifa Alhaj Babiker
Abstract: Background: Stroke is the second leading cause of death after ischemic heart disease; IL-16 is identified as a pro-inflammatory cytokine that is a key element in the ischemic cascade after cerebral ischemia. In this study IL-16 gene was investigated for the first time in Sudanese ischemic stroke patients with special emphasis for detecting the rs11556218 T/G which was reported as more frequent in ischemic stroke. This polymorphism was reported to be associated with a series of inflammatory or autoimmune diseases, including Crohn's disease, allergic contact dermatitis, endometriosis, asthma, Graves' disease, and systemic lupus erythematosus (SLE). Objectives: this study aimed to investigate the association between the IL-16 gene polymorphisms and of ischemic cerebral stroke in Sudanese patients as well as the association of this polymorphism with different ischemic strokes risk factors. Material and Methods: This is a cross-sectional study conducted at the National Center for Neurological Sciences (NCNS), Khartoum, Sudan, during the period of March 2016 to September 2017. Structural questionnaire was designed to collect the demographic and clinical data concerning each participant. Hematological tests including platelets count and D-dimer were conducted. The genomic DNA was extracted from blood samples collected from the ischemic stroke patients and controls, and then IL-16 gene was amplified using polymerase chain reaction (PCR). Thereafter, 42 IL-16 PCR products were sent for sequencing to Macrogen Europe Laboratory and BGI-solutions Hong Kong Co. Ltd. Respectively. The data were analyzed using SPSS version (21), with reference p.value 5% (0.05) was considered to be statistically significant. The descriptive statistics were obtained for quantitative variables while (frequency, percent) for qualitative variables. The independent sample t-test used to test the difference between the quantitative variables among the two groups. The test of goodness of fit and the test of independency using chi-square were performed to test the relationship between qualitative variables. The sequencing results were analyzed using different bioinformatics soft-wares and tools. The obtained sequences, aligned using BioEdit- ClustalW software with a normal sequence from GenBank retrieved from National Center of Biotechnology Information (NCBI) were examined for the presence of polymorphisms. Results: One hundred and ten of ischemic stroke patients were included and 49 apparently healthy individuals were selected as controls. Males constituted 52.7% of the study population. The most affected age group was 65-82 years (48 patients), followed by 48-64 years (32 patients) constituting (44, 29%) respectively (P = 0.000). The studied risk factors associated with ischemic stroke have shown that hypertension and heart disease were significantly associated with ischemic stroke (P =0.000, 0.018) respectively. D.dimer was performed to 54 ischemic stroke patients, 83% have D. dimer ≥0.3 mg/l (P=0.000). High level of D.dimer was detected in 35.6% of hypertensive ischemic stroke patients while 8.9% of patients with heart diseases developed abnormal D.dimer results. The mean of platelets count was 231 for patients and 258 for control group (P = 0.059). The polymorphism rs11556218 T/G was detected in 75% of the patients (50% were having TG allele, GG allele was found in 25%). Mutation in IL-16 gene was associated with ischemic stroke risk factors including D. dimer (OR=3.5) and hypertension (OR=1.6). Moreover, it has significant relationship with ischemic stroke (OR=3). Conclusion: These findings indicated that the rs11556218 T/G can be used as indicator for ischemic cerebral stroke. Also this polymorphism is significantly associated with D.dimer and Hypertension.
URI: http://khartoumspace.uofk.edu/123456789/27116


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