University of Khartoum

Antiviral Activity of Acacia nilotica Against Influenza AVirus

Antiviral Activity of Acacia nilotica Against Influenza AVirus

Show full item record

Title: Antiviral Activity of Acacia nilotica Against Influenza AVirus
Author: Gassab, Mona Timan Idriss
Abstract: Background: Human influenza is a seasonal disease associated with significant morbidity and mortality.The emergence of drug-resistant virus strains highlights the urgent need to develop novel antiviral drugs with alternative modes of action. Anti-flu Traditional Chinese Medicine (TCM) has played a significant role in fighting the virus pandemic. In Sudan, Acacia nilotica is a commonly used ingredient in many therapeutic remedies, either alone or in conjunction with other natural substances. Evidence suggests that Acacia nilotica is associated with a variety of pharmacological activities. Object of this studywas to investigate the influence of Acacia niloticamethanolic extract on the infectivity of influenza viruses in Madin–Darby Canine Kidney (MDCK) cells, and its possible in vitro mechanism(s) of action. Methodology: The fruit husk of Acacia niloticawas collected from Sudan and extracted with70% methanol. The crude extract was screened for its cytotoxicity against MDCK cell line by crystal violet assay and Water Soluble Tetrazolium-1 (WST-1) assay. Antiviral properties of Acacia niloticaextract was determined by cytopathic inhibition assay, virus yield reduction assay (plaque assay), nuclear export inhibitor and western blotting assay. To assess the possible mechanism of action, time of addition assay was performed targeting different sites in virus life cycle. Result:Acacia nilotica has been found to have anti-influenza-virus activity, and both pre-incubation of virus prior to infection and post-exposure of infected cells with Acacia nilotica extracts significantly inhibited virus yields. Influenza-virus-induced hemagglutination of chicken red blood cells was inhibited by Acacianilotica extract, suggesting that Acacia nilotica can inhibit influenza A virus infection by interacting with the viral hemagglutinin. Furthermore, Acacianilotica extract significantly affects nuclear transport of viral nucleoprotein (NP). Conclusion: The antiviral activity of Acacia niloticaextracts indicates that a component or components of these extracts possess anti-influenza virus properties. To the best of our knowledge, this study revealed for the first time that Acacia nilotica extract can inhibit both viral attachment and replication and offers new insights into its underlying mechanisms of antiviral action.
URI: http://khartoumspace.uofk.edu/123456789/27122


Files in this item

Files Size Format View

This item appears in the following Collection(s)

Show full item record

Share

Search DSpace


Browse

My Account