University of Khartoum

Anti-tyrosinase, total phenolic content and antioxidant activity of selected Sudanese medicinal plants

Anti-tyrosinase, total phenolic content and antioxidant activity of selected Sudanese medicinal plants

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dc.contributor.author Muddathir, A.M.
dc.contributor.author Yamauchi, K.
dc.contributor.author Batubara, I.
dc.contributor.author Mohieldin, E.A.M.
dc.contributor.author Mitsunaga, T.
dc.contributor.other Department of Horticulture en_US
dc.date 2017
dc.date.accessioned 2019-10-19T11:39:25Z
dc.date.available 2019-10-19T11:39:25Z
dc.date.submitted 2019
dc.identifier.uri http://khartoumspace.uofk.edu/123456789/27173
dc.description.abstract The flora of Sudan is relatively rich in medicinal plants and represents an important component of traditional medicine. Fifty methanolic extracts of selected Sudanese medicinal plants were evaluated for their in vitro tyrosinase inhibitory effect, antioxidant activity and total phenolic content (TPC). The standard method of antioxidant evaluation, ferric reducing ability of plasma (FRAP), was employed to determine the antioxidant activity while the enzyme based tyrosinase inhibition was used for the anti-tyrosinase activity. Acacia nilotica (pods, bark) and Acacia seyal var. seyal (wood) demonstrated comparable anti-tyrosinase inhibitory activity using L-tyrosine as substrate (08.61, 10.47 and 10.77 μg/ml respectively) to Kojic acid (10.02 μg/ml) which was used as a positive control. A. nilotica (bark) and Acacia seyal var. fistula (bark) exhibited good tyrosinase inhibitory activity using L-DOPA as substrate (IC50: 31.93, 36.32 μg/ml) compared to positive control (IC50: 37.63 μg/ml). The results revealed significant differences in TPC between plants extracts. The highest level of phenolic content was found in Terminalia brownii (bark; 46.02 μg GAE/mg) while the lowest was in Ziziphus spina–christi (fruits; 09.63 μg GAE/mg). The study indicated significant differences in total antioxidant capacity between the extracts. Terminalia laxiflora (wood), A. nilotica (pods, bark), T. brownii (bark), A. seyal var. seyal (bark), Khaya senegalensis (bark), T. brownii (wood) Combretum hartmannianum (bark), Polygonum glabrum (leaves), Z. Spina-christi (bark) and Guiera senegalensis (leaves) extracts displayed the high antioxidant equivalent concentration (EC) values. A. nilotica (pods, bark) expressed promising activity that warrant further research since it has high tyrosinase inhibitory activity, antioxidant activity and could be a good source of phenolic compounds. To the best of our knowledge, this is the first data presenting comprehensive data on antityrosinase, TPC, antioxidant activity of the Sudanese medicinal plants en_US
dc.language.iso en en_US
dc.publisher University of Khartoum en_US
dc.subject Sudanese medicinal plants ;Phenolic content ;FRAP; Tyrosinase inhibition; Acacia nilotica en_US
dc.title Anti-tyrosinase, total phenolic content and antioxidant activity of selected Sudanese medicinal plants en_US
dc.type Publication en_US
dc.Faculty Agriculture en_US

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