University of Khartoum

Patterns of Hepatitis B Virus Genotypes Among Sudanese With Chronic Hepatitis B Virus Infection

Patterns of Hepatitis B Virus Genotypes Among Sudanese With Chronic Hepatitis B Virus Infection

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Title: Patterns of Hepatitis B Virus Genotypes Among Sudanese With Chronic Hepatitis B Virus Infection
Author: Adam, Eman Yassin Salih
Abstract: Background: Hepatitis B virus (HBV) infection is a global health problem that usually cause chronic liver infection with progression to liver cirrhosis and hepatocellular carcinoma, Chronic HBV infection affects more than 240 million people worldwide of which 65% million reside in Africa. Sudan is classified among the countries with high hepatitis B surface antigen prevalence. HBV is a members of the hepadnaviridae family, its small circular DNA genome contain genes for antigenic components including hepatitis B surface antigen (HBs Ag), hepatitis B core antigen (HBc Ag) and hepatitis B e antigen (HB e Ag). Design: This was a prospective, cross-sectional and analytical study. Settings: Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan. Samples were collected from blood donor units, blood banks, Central Blood Bank, Khartoum, Gadaref and Kasala states. Objectives: to determine genotypes and genetic variants of HBV among Sudanese HBs Ag reactive individuals / patient from different geographic site in Sudan in central and eastern states. Materials and Methods: Five hundred and thirty five samples were taken from HBV patients and consenting blood donors/patient from central and eastern states, Sudan. Sera were tested for HBs Ag using Rapid Immuno-chromatographic (ICT)/ELISA. DNA was extracted from serum samples using the guanidine extraction method. DNA samples were amplified by nested/ multiplex PCR for detection of genotypes A-F. In the first round, universal outer primers, two second round PCR reactions were performed using universal inner primers specific for genotypes A-C and another set that identifies genotypes D-F. The products of the second round were then run on an a agarose gel electrophoresis system and compared to a 50bp DNA ladder to determine the different HBV genotypes. Results: All samples were reactive in Rapid Immuno-chromatographic (ICT)/ELISA tests and had HBV DNA traces in their sera. The majorities (454/535; 85%) of the samples were identified as genotype A. Ten percent (54/535) were identified as genotype E, while three percent (3%, 15/535)were identified as genotype, while2%(12/535) showed multiple recombination of genotype A,E&D. Genotypes D and E were always detected combined with genotype A. Mixed genotypes were detected in Eastern Sudan samples. Conclusion: HBV genotype patterns in Sudan showed a mixture of A, D and E genotypes separately or in recombination reflecting previous and ongoing migration waves from surrounding territories.
Description: 105 Pages
URI: http://khartoumspace.uofk.edu/123456789/27327
Date: 2018


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