University of Khartoum

Identification of Leishmania donovani from Infected Dogs at A Dormant Focus of VL in Blue Nile State, Sudan

Identification of Leishmania donovani from Infected Dogs at A Dormant Focus of VL in Blue Nile State, Sudan

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Title: Identification of Leishmania donovani from Infected Dogs at A Dormant Focus of VL in Blue Nile State, Sudan
Author: Osman, Omran F.; Shamboul, Khalid M.; El Bagir, Ammar M.; El Sayed, Makarem O.; Saeed, Sara A. K.; Abdalla, Hanan
Abstract: The objectives of this study were to investigate the presence of Leishmania donovani in the susceptible reservoir/host in a dormant focus of Visceral Leishmaniasis endemic area and to define the prevalence of L. donovani in dogs in the district. The importance of the study stems from the fact that no epidemiological studies were conducted since the tragic epidemic of VL in 1956. In this study, 47 dogs were screened for infection with Leishmania donovani. Peripheral blood samples were taken from the tip of the ear of each dog and subsequently screened by the Polymerase Chain Reaction (PCR) and Direct Agglutination Test (DAT) test. DAT on blood samples detected anti-Leishmania antibodies in 8 out of the 47 investigated dogs. Two PCR assays were applied, one primer pairs target genomic DNA and the another target kinetoplast (mitochondrial) DNA. Both methods targeting kinetoplast DNA (RV1-RV2) and genomic DNA (Pia1-Pia 2) detected the presence of Leishmania DNA in all of serologically positive dogs. Moreover, six serologically negative dogs were found PCR positive. Eight out of 47 blood samples tested were PCR positive, giving an amplification product of 145 and 100 bp. The findings of the present study may suggest that the natural transmission of the parasite is active in the area although no cases has been reported in the recent years, therefore more attention should be taken to monitor VL transmission to control and prevent future outbreaks.
URI: http://khartoumspace.uofk.edu/123456789/27822


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