In vitro Shoot Initiation and Multiplication of Jojoba Simmondsiachinensis (Link.) Schneider

Abstract

In vitro clonal propagation of a one year-old jojoba tree through axillary shoot proliferation was achieved. Nodal segments, approximately 3 cm long; each including one node with two axillary buds; were used as explants. The nodal segments were cultured on MS medium supplemented with three concentrations of BA (1.0, 2.0, or 3.0 mg L-1) and NAA (0.1, 0.2, or 0.3 mg L-1). Different growth parameters such as number of buds, length of primary shoot (cm), and number of leaves per primary shoot were recorded. The combination of 1.0 mg L-1 BA and 0.1 mg L-1 NAA exhibited the minimum time for bud sprouting (26 days), followed by the combination2.0 mg L-1 BA and 0.2 mg L-1 NAA (29 days). While the combination of 3.0 mg L-1 BA and 0.3 mg L-1 NAA exhibited the maximum time for bud sprouting (31 days). The results revealed that the combination of 1.0 mg L-1 BA and 0.1 mg L-1 NAA resulted in minimum time for bud sprouting, maximum length of shoot, high number of shoots per explant and greater number of leaves per primary shoot.