University of Khartoum

Sex Determination in Plants

Sex Determination in Plants

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Title: Sex Determination in Plants
Author: Mohammed, Ismail A.; Mohammed, Israa A.; Osman, Fatima M.
Abstract: Date palm is one of the most important economical crops in world. Sex determination of date palm in early stage is a prerequisite for breeding and cultivation. DNA was extracted from ten seedlings and five male and female plants using CTAB method. In this study a total of eight primers, six RAPD and two ISSR primers were examined for their validation in sex determination of date palm genotypes growing in Sudan. PCR amplification was performed using these primers. Among these primers only four RAPD primers OPA02, OPJ-09, RD A02 and RD A21 were amplified male specific band with size of 1000, 1100, 1000 and 1400 pb respectively. The specific bands were observed clearly among all male genotypes and absent in female samples unknown samples irrespective of genotypes. Those primers are reproducible, effective and highly trusted in the identification of the date palm in the seedling stage.
URI: http://khartoumspace.uofk.edu/123456789/27920


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