University of Khartoum

Enhancement of drought tolerance in Jatropha curcas L. by introducing glycine betaine and NF-YB6 genes

Enhancement of drought tolerance in Jatropha curcas L. by introducing glycine betaine and NF-YB6 genes

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Title: Enhancement of drought tolerance in Jatropha curcas L. by introducing glycine betaine and NF-YB6 genes
Author: Mohammed, Ismail A.
Abstract: Jatropha curcas L. (jatropha) is an oilseed crop that has ability to grow in marginal and semimarginal lands. In this study, two kinds of DNA constructs were introduced into jatropha separately by the Agrobacterium method, in order to enhance its drought tolerance.The first one contains a set of cyanobacterium genes, GSMT (glycine-sarcosine methyltransferase) and DMT (sarcosine-dimethylglycine methyltransferase), encoding enzymes to catalyze synthesis of glycine betaine, which works as an osmoprotectant in plant cells. The second one contains a jatropha transcriptional factor gene, JcNF-YB6 (Jatropha curcas Nuclear Factor Y b subunit 6), which was identified in this study. Both the constructs were designed to overexpress the genes by the CaMV 35S promoter. Nineteen putative GSMT-DMT transgenic plants were acclimatized, and their molecular analysis showed that two plants had the GSMT gene. The putative JcNF-YB6 transgenic plantlets were developed in the shoot regeneration media.
URI: http://khartoumspace.uofk.edu/123456789/27926


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