University of Khartoum

Rapid detection of Peste des Petits Ruminants (PPR) virus antigen in Sudan by agar gel precipitation (AGPT) and haemagglutination (HA) Tests

Rapid detection of Peste des Petits Ruminants (PPR) virus antigen in Sudan by agar gel precipitation (AGPT) and haemagglutination (HA) Tests

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dc.contributor.author Osman, Nussieba A.
dc.contributor.author A/Rahman, Mahasin E
dc.contributor.author Ali, A. S.
dc.contributor.author Fadol, M. A.
dc.date.accessioned 2014-05-20T08:36:21Z
dc.date.available 2014-05-20T08:36:21Z
dc.date.issued 2008
dc.identifier.citation Osman,Nussieba A.etd.(2008).Rapid detection of Peste des Petits Ruminants (PPR) virus antigen in Sudan by agar gel precipitation (AGPT) and haemagglutination (HA) Tests.artecile.6pages en_US
dc.identifier.uri http://hdl.handle.net/123456789/497
dc.description article 6pages en_US
dc.description.abstract AGPT and HA tests were employed for rapid diagnosis of PPRV infection in sheep and goats in Sudan. Forty lymph nodes and spleen samples from suspected cases of PPR in both sheep and goats were examined by AGPT and HA tests for detection of PPRV antigen. Viral antigen was detected from (77.5%) of the samples tested by AGPT and (92.5%) tested by HA test. The results of both tests revealed that HA test was more sensitive than AGPT for detection of PPRV antigen (Kappa statistics 0.4366). Another advantage of the HA test over AGPT was that it can differentiate PPRV from RPV. Thus the HA test represents a quick, easy, simple, cheap and reliable confirmatory test for the diagnosis of PPR and differential diagnosis of PPRV and RPV. The HA test was carried out using chicken, goat and pig RBCs. Chicken RBCs were found to be the most sensitive for detection of PPRV antigen, followed by goat then pig RBCs. The HA time when using chicken RBCs was 20–25 minutes, using goat RBCs was 25–30 minutes and using pig RBCs was 40– 45 minutes. The distribution of PPR infection infour different regions of Sudan was investigated. en_US
dc.subject PPRV en_US
dc.subject Haemagglutination (HA) test en_US
dc.subject Agar gel precipitation test (AGPT) en_US
dc.subject RBCs en_US
dc.title Rapid detection of Peste des Petits Ruminants (PPR) virus antigen in Sudan by agar gel precipitation (AGPT) and haemagglutination (HA) Tests en_US
dc.type Book en_US

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