University of Khartoum

Characterization of Leishmania amastigote and axenic form antigens

Characterization of Leishmania amastigote and axenic form antigens

Show simple item record M. Mukhtar, Maowia Awad, Aymen 2015-03-04T09:13:47Z 2015-03-04T09:13:47Z 2005
dc.description.abstract This study was designed to identify amastigote and axenic form antigens of leishmania major and leishmania donovani isolates. Two in vitro methods were used to transform the parasite isolates from promastigote to amastigotes and amastigote like (axenic) form. The first was in vitro infection of macrophages cell line J774 with leishmania promastigote, at 37o C with 5% CO2. The second was the culture of promastigote at 37o C with low pH (5.5), and 5-10% CO2, for axenic form transformation. Proteins were extracted from promastigote, amastigote, axenic form and J774 macrophages, simultaneously infected with L.major and L.donovani. Proteins were fractionated using 12% SDS PAGE. Antigens were detected using both dot blot & western blot techniques. PCR method was performed for detection of leishmania parasites in infected J774 macrophages. L. major was faster in infectivity of macrophages cell line than L. donovani (duration time for L. major 3-5 days, for L. donovani 7-9 days). Shared protein bands ranging from 26-116 kDa were detected by SDS PAGE in all stages. Dot blot technique revealed positive results, while western blot detected a unique antigen band of 16 kDa in amastigote from infected macrophages. PCR results were positive for both isolates showing that co infection is possible, and no signs of genetic recombination at kDNA were detected in macrophages simultaneously infected by L.major and L.donovani. en_US
dc.publisher University of Khartoum en_US
dc.subject Leishmania parasite surface molecules en_US
dc.title Characterization of Leishmania amastigote and axenic form antigens en_US
dc.type Thesis en_US

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