University of Khartoum

Genotypes Distribution of Plasmodium Falciparum Erythrocyte Binding Antigen (Eba-175) Gene in Sudanese Malaria Patients

Genotypes Distribution of Plasmodium Falciparum Erythrocyte Binding Antigen (Eba-175) Gene in Sudanese Malaria Patients

Show full item record

Title: Genotypes Distribution of Plasmodium Falciparum Erythrocyte Binding Antigen (Eba-175) Gene in Sudanese Malaria Patients
Author: Adam,Ahmed Adlan Mohammed
Abstract: Background: In many Plasmodium falciparum (P. falciparum), strains the invasion process requires an interaction between merozoite ligands and the sialic acid residues of glycophorin A (GPA) on the erythrocyte surface. It was found that the majority of P. falciparum merozoites use the erythrocytic GPA receptor to invade RBCs. The Erythrocyte Binding Antigen (EBA-175) has highly divergent allelic segments (C seg and F seg). There was a study showed that the presence of C segment was not associated with severe malaria but confer a higher risk of fatal disease. Design: This study was cross-sectional study, hospital and village based. Setting: General and paediatric Madani Hospitals, Al-Emam Al-bukhari primary school, Sennar malaria centre, and Um-Salala village were surveyed in the time from the 2nd of November to the 1st of September 2007 and 2009. Objective: • To determine the frequencies of the dimorphic allele segments of the EBA-175 gene of the P.falciparum merozoite among malaria patients in Sudan. • To investigate whether this dimorphism has a significant association or impact on the clinical manifestation of the malaria disease. Methods: Blood samples were collected from 339 malaria patients from the three regions. 20 =549;l Peripheral blood dried on filter paper in addition to 2 ml whole venous blood for thin and thick blood film for parasite identification and count, ICT, Hb, Random Blood Glucose level, and PCR. Results: The Genotyping results for all samples showed frequencies of different alleles as follow 41.0% for F allele, 51.0% for C allele and 8.0% with mixed infection (allele F+C). F and C alleles were showed significantly different distributions in different geographic areas (p-value= 0.00). The correlation between different malaria phenotype manifestations and genotypes revealed that the severity of the diseases correlated with high frequency of C allele (62.9%), at least in Sennar region. Conclusion: This study showed that significant differential distribution of F and C fragments in different geographical areas endemic with Malaria which could explain the different pattern of malaria in Um-Salala village in the East and cities in central Sudan. No significant association was observed between F and C alleles and different Malaria phenotypes, this association was found in Sennar area but the samples size was too small so this results needs to be verified
URI: http://hdl.handle.net/123456789/6326
Date: 2010


Files in this item

Files Size Format View

This item appears in the following Collection(s)

Show full item record

Share

Search DSpace


Browse

My Account