University of Khartoum

Pregnancy-associated malaria in Greater Khartoum: prevalence and immunological aspects

Pregnancy-associated malaria in Greater Khartoum: prevalence and immunological aspects

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Title: Pregnancy-associated malaria in Greater Khartoum: prevalence and immunological aspects
Abstract: Abalaria continues to threaten more than ٤١٪ of the world’s population. Pregnancyassociated malaria is a well-documented cause of morbidity and mortality to mothers and their developing fetuses in sub-Saharan Africa. The presence of certain molecules like chonroitin sulphate A (CSA) and the predominance of Th٢ cell immune responses contribute greatly to the increased incidence, pathogenecity and morbidity of pregnancyassociated malaria. A prospective, longitudinal hospital based study was conducted in a central maternity referral hospital in Greater Khartoum during the period of July ٢٠٠٣-December ٢٠٠٤ to determine the prevalence of pregnancy-associated Plasmodium falciparum infection and immune profiles of the mothers and their newborns. Pregnancy outcome of mothers with pregnancy associated malaria was also investigated. Volunteers pregnant women consented to participate were interviewed and examined clinically. Blood samples were collected for thin and thick blood films, peripheral and cord blood mononuclear cells were collected for culture and stimulated with MSP١١٩. Anti-MSP١١٩ IgG antibodies were measured using bulk Enzyme Linked Immunosrbent Assay (ELISA) with determination of cut-off points. cytokine profiles of mothers and their babies were determined using commercial ELISA kits. Samples were also collected for DNA extraction and amplification using specific P. falciparum primers. Following DNA amplification P. falciparum multiplicity of infection was calculated.
URI: http://hdl.handle.net/123456789/6370
Date: 2006


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