University of Khartoum

Interleukin-10 (IL-10) gene polymorphisms in development of Post Kala-azar dermal leishmaniasis (PKDL)

Interleukin-10 (IL-10) gene polymorphisms in development of Post Kala-azar dermal leishmaniasis (PKDL)

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dc.contributor.author Farouk, Shimaa
dc.contributor.author Salah Eldin, Hiba
dc.contributor.author El-Tayeb, Muntasir
dc.date.accessioned 2015-03-04T11:37:47Z
dc.date.available 2015-03-04T11:37:47Z
dc.date.issued 2007-05
dc.identifier.uri http://hdl.handle.net/123456789/6377
dc.description.abstract goal of this study to determine if there is an association of IL-10 promoter gene polymorphisms with Post Kala-azar dermal leishmaniasis (PKDL) in the highly susceptible Masalit tribe from three neighboring villages Um-Salala, EL-Rugab and Ali babkir. DNA was collected from 85 families consist of 51 trios and 34 pairs. - 592C/A, - 819C/A and -1082A/G of IL-10 promoter gene polymorphisms were genotyped using PCR-RFLP and ARMS-PCR techniques. The data were tested to their fit to Hardy- Weinberg Equilibrium and the polymorphisms were analyzed using Transmission Disequilibrium Test (TDT) and Haplotype Based Score Test to test the association to the disease. All polymorphisms were in Hardy-Weinberg Equilibrium. TDT analysis showed No evidence of association between IL-10 -592C/A, -819C/T and -1082A/G and susceptibility to PKDL (p>0.05, 0.7855, 0.7815, and 0.5862, respectively). Haplotype Based Score Test showed border significant between -592C/T and -1082A/G (p=0.053). All eight haplotype probabilities of three target loci were observed in Masalit tribe with higher frequency of GCC and ATA haplotype 32% and 30% respectively en_US
dc.publisher University of Khartoum en_US
dc.subject Leishmaniasis,Post –kala-azar dermal leishmaniasis (PKDL),Linkage Disequilibrium (LD),Transmission Disequilibrium Test (TDT), en_US
dc.title Interleukin-10 (IL-10) gene polymorphisms in development of Post Kala-azar dermal leishmaniasis (PKDL) en_US
dc.type Thesis en_US

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