University of Khartoum

THE POSSIBLE ROLE OF EPSTIEN- BARR VIRUS (EBV) IN THE DEVELOPMENT OF BREAST CARCINOMA IN SUDANESE PATIENTS

THE POSSIBLE ROLE OF EPSTIEN- BARR VIRUS (EBV) IN THE DEVELOPMENT OF BREAST CARCINOMA IN SUDANESE PATIENTS

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Title: THE POSSIBLE ROLE OF EPSTIEN- BARR VIRUS (EBV) IN THE DEVELOPMENT OF BREAST CARCINOMA IN SUDANESE PATIENTS
Author: ADAM, ZEINAB; ELTAYEB, MUNTASER; HAMAD, KAMAL
Abstract: Breast cancer is a common cancer in Sudanese women; its main causes remain unknown. Numerous etiological factors have been proposed, but they account for only a small percentage of the total cases. Viral pathogens have not been much explored, but recent interest has focused on Epstein-Barr virus (EBV). A sequence polymorphism at codon 72 of the p53 gene results in either a proline or an Arginine and may induce different functional activities. This polymorphism has been shown to have varying ethnic and geographical distribution among Sudanese. In this current study DNA from 92 breast cancer tissue samples, collected from different hospitals in Khartoum were analyzed. Two aims were investigated: (1) the association between Epstein - Barr virus infection and the development of breast carcinoma in Sudanese Patients. (2) Whether there is a correlation between polymorphism in codon 72 of p53 and the development of breast carcinoma in relation to EBV. DNA was extracted from the tissue samples of breast carcinoma and Polymerase chain Reaction (PCR) technique was used. EBV was found in 53.3% of the tumors and 24% among controls which indicate that EBV is mostly restricted to the tumors (P=0.0055). The proportions of p53 codon 72 genotypes were found to be 78.3% Arginine homozygous, 4.3% proline homozygous and 17.4% heterozygous among patients with breast cancer and 21.7% Arginine homozygous 24.5% proline homozygous and 53.8 % heterozygous among the control population (P= 0.001). The Frequency of the two alleles in a series of EBV-positive samples was compared with the frequency in the EBV-negative group. The p53 Arg allele alone was detected in (87.5%) of the EBVpositive group compared with (69.7%) of the EBV-negative group (P= 0.53). The Pro homozygous alone genotype and Arg/ Pro genotype was found in (14.5%) of the EBV-positive group, compared with (30.2%) of the EBV-negative group (P= 0.15). Hence, an individual homozygous for p53 Arg are not differing in risk to develop EBV associated breast carcinoma than would an Arg/ Pro heterozygote or a Pro homozygote. Statistical analysis suggested a strong association of EBV with breast cancer and between the Arg/Arg genotype and breast cancer. In Conclusions these results suggest that in Sudanese population there may be a correlation between the presence of EBV and the developments of breast carcinoma and between p53 codon 72 Arginine polymorphism and the development of breast cancer independently.
URI: http://hdl.handle.net/123456789/6385
Date: 2007-08


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