University of Khartoum

Antimicrobial And Wound Healing Activity

Antimicrobial And Wound Healing Activity

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Title: Antimicrobial And Wound Healing Activity
Author: Elnazeir, Hajer
Abstract: The chloroform, methanol and aqueous extracts of the Guiera senegalensis and Kigelia africana, were tested for their antimicrobial activity against five standard bacteria ,two standard Gram-positive bacteria (Bacillus subtilis NCTC 8236 and Staphylococcus aureus ATCC 25923), three standard Gram-negative bacteria (Escherichia coli ATCC 25922, Proteus vulgaris ATCC 6380 and Pseudomonas aeruginosa ATCC 27853) and the two fungi (Aspergillus niger ATCC 27853 and Candida albicans ATCC 7596), using the cup plate agar diffusion method. The aqueous and methanolic extracts of the two plants proved to be more effective against the bacteria and fungi tested than the chloroform extracts. The minimum inhibitory concentrations (MICs) of the most active extracts against the standard bacteria and fungi were determined using the agar plate dilution method. The antimicrobial activity of six reference drugs were determinated against the seven tested Gram positive ,Gram negative bacteria and fungi and compared their activity with the activity of the tested plant extracts . The most active extracts against the standard bacteria were selected, and III then they were tested against 100 clinical isolates collected randomaly from the National Health Laboratory, Khartoum Educational Hospital and Omdurman Educational Hospital. Kigelia africana is one of the most widely used medicinal plants in Sudan, and is employed in numerous traditional preprations. In this study the wound healing effect of Kigelia africana fruit extract was investigated on open skin wound model on rats. 15 Swiss Wistar Albino rats of either sex weighing (80-100 gm) were used during the study Hair of the lower back and right flank of the animal was completely shaved. Full thickness circular excision wound 1 cm in diameter was made on the shaved area. Methanolic extract of Kigelia africana fruit was prepared. Ointment of 2% (w/w) extract in polyethylene glycol was prepared. Fucidin ointment was used as standard healing agent; both ointments were applied twice daily. One trail was performed using three groups of rats. Treated groups were compared with non-treated groups. Healing was determined by reduction in the size of wound area. The results of this study confirmed that the 2% Kigelia africana ointment is a potent healing agent even better than the tested Fucidin ointment.
Description: 150 Pages
URI: http://hdl.handle.net/123456789/7816
Date: 2015-03-31


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