University of Khartoum

Evaluation of Parricidal Activity Of Some Sudanese Medicinal Plants Against Experimental Transom Evans Infection

Evaluation of Parricidal Activity Of Some Sudanese Medicinal Plants Against Experimental Transom Evans Infection

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Title: Evaluation of Parricidal Activity Of Some Sudanese Medicinal Plants Against Experimental Transom Evans Infection
Author: HUSSEIN, SAMIA
Abstract: Plants have always been among the common sources of medicaments. In this study three different plants from different areas of Sudan had been studied for their trypanocidal activation. Tinospora bakis, Argimone mexicana and Aristolochia bracteolata were evaluated for in vivo activity against Trypansoma evansi infection in rats. Five groups of 5 rats each aged 5-7 weeks, average weight 150 gram were used. The plant extract was administered orally at dose rate of 500 mg /kg BW for the chloroformic extract and 500 mg/kg BW and 250 mg/kg BW for the methanolic extract. The result was compared to Cymerlarsan which was given at the recommended dose rate of 2.5 mg/kg BW subcutaneously. The parasitaemia in each rat was followed for 30 days after treatment. Blood was collected every week for analysis. For the toxicity effect of the plant extract three groups of 5 rats each where given only the plant extract with a forth group left as a control. The mortality was observed for 30 days. Results showed that Tinospora bakis extract was found to be effective in cleaning the parasite for a considerable time and extending life-spam of the treated rats. 15 Argimone mexicana on the other hand was found to be more effective as for both the chloroformic and methanolic extracts were effective in clearing or reducing the parasitaemia. Aristolochia bracteolata chloroformic extract gave a very good trypanoscidal effect with the where clearance of the parasite was 100%. Whereas the methanolic extract gave a limited trypanocidal effect. Tinospor bakis was found to be non toxic as the treated rats were active and healthy throughout the experiment. Histopahtological examination showed no significant changes in tissues except for mild to moderate congestion in liver and spleen. The cholroformic extract of Argimone mexicana was found to be toxic with 60% death rate accompanied by degenerative changes in liver with hepatocellular necrosis. The serum total protein levels, GOT ad GPT was found to be increased. The chloroformic extract of Aristolochia bracteolata was found to be more toxic with 100% death rate and on histopathological examination, gromulonephritis was seen and the liver also showed heaptocellular necrosis. There was a significant increase in total protein, GPT levels in blood. Serum analysis resulted in that only the infected and untreated group of rats showed significant biochemical changes. There was increase in total protein, urea and 16 creatinine, accompanied with decrease in the concentration of albumin, potassium, sodium, magnesium, copper and zinc.
Description: 154 page
URI: http://hdl.handle.net/123456789/7958
Date: 2015-04-01


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