University of Khartoum

Extraction, Purification and Formulation of Beef Insulin

Extraction, Purification and Formulation of Beef Insulin

Show full item record

Title: Extraction, Purification and Formulation of Beef Insulin
Author: Elimam, Abdalla
Abstract: In this study fresh bovine pancreatic glands trimmed off fat were frozen rapidly at 20 o C. The frozen glands were chopped into small pieces and then homogenized and centrifuged after adjustment of pH and then filtered. Crude insulin is precipitated from the filtrate and dissolved in acetic acid. The insulin was again precipitated iso-electrically and crystallized using zinc acetate. After the process of extraction,the process purification and formulation of beef insulin was continued. Furtherly, the two scales formulated were subjected to quality control test. The study was done for both scales and formulation, initially the study includes yield percent for all samples, their zinc content (mg/100 U); number of nitrogen percent obtained from sample and study of potency carried out by HPIC. The purity test of both scales basedon the migration of changed particles dissolved or dispersed in an electrolytic solution under the action of an electric field, under precisely determined operational conditions, indicates the purity of the studied sample. In the study of insulin assay by blood-sugar method, to determine the dilution to be injected into rabbits showed the number of units suitable to prepare the dose of standard and assay dilution. Also the calculation of individual and total response and the effect of insulin were carried out. In another part of the study of sterility test the method which used was a direct transfer method, proceed as directed under procedure. For e extremely large devices, immersed those portions in a volume of medium sufficient to achieve complete immersion of those portions. Two medium were used thioglycolate and soybean tests. In one study of stability test the different formula stored at 4 o C for six month and then the quality control test were carried out: Determination of zinc insulin determination of Nitrogen content sterility test and finally purity test (electrophoresis). In addition the study includes well supported data showing both the annual amount of the imported insulin and the possible quantity of insulin that could be produced locally as a bi-product utilizing the slaughtered cattle in Sudan. Furthermore, the comparison of the prices of the two product showed the economical feasibility of the locally manufactured insulin in the country.
Description: 190 page129 page
URI: http://hdl.handle.net/123456789/8047
Date: 2015-04-01


Files in this item

Files Size Format View

This item appears in the following Collection(s)

Show full item record

Share

Search DSpace


Browse

My Account