University of Khartoum

Detection of mutations in drugs resistance genes Mycobacterium tuberculosis in Khartoum state

Detection of mutations in drugs resistance genes Mycobacterium tuberculosis in Khartoum state

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Title: Detection of mutations in drugs resistance genes Mycobacterium tuberculosis in Khartoum state
Author: Suliman, Ghada
Abstract: In this study, the molecular biological technique was used to study the drugs resistance in 120 samples of Mycobacterium tuberculosis(M. tb) isolated from Khartoum State. A systematic random samples were isolated from patients with sputum smear positive for acid-fast bacilli (AFB), from relapsed, treatment failed, defaulted, and new cases under treatment for more than two months. Sputum samples were initially subjected to standard microscopic and cultural techniques for tentative identification, then confirmed using dna J gene analysis. Ninety-five out of the 120 samples revealed positive growth on L J media incubated at 37°C for up to 6 weeks. By using dnaJ gene analysis, 50 (52.6 % ) out of 95 were found to be Mycobacterium tuberculosisand four (4.2 %) belong to mycobacteria other than tuberculosis (MOTT ).The Mycobacterium tuberculosis( 50 samples) were subjected to PCR-based dot blothybridization technique by using radio-labeled P 32 to detect mutations in different loci in six genes (katG 315, rpoB 531, rpsL 43, rrs 513 & rrs 491 and embB 306 ) associated with drug resistance to isoniazid, rifampicin, streptomycin and ethambutol respectively. Mutations genes were identified in 12% ( katG 5 gene ), 8% (rpoB gene ), 20% (rpsL),10 % (rrs genes ) and 6 %.(embB gene ) associated with isoniazid, rifampicin, streptomycin and ethambutol respectively, and 4% were multi-drug resistance tuberculosis ( MDR-TB). Among the fifty tuberculosis patients, Thirty nine (78%) of them had problematic tuberculosis distributed as follow: The treatment failed cases (36 %) had mutations associated with four genes, katG (5.6 %), rrs (11.1 %), rpsL (16.7 %), and rpoB (5%) The relapsed cases (28%) had mutations associated with tow genes, katG gene (14.3 %) and rrs gene (21.4 %). The defaulted cases (14 %) had mutations associated with four genes, katG (28.6 %), rrs ( 42.9 % ), rpsL (14.3 % ), and embB (42.9% ). Twenty two percent of newly diagnosed patients were found to be resistant to rifampicin and also resistance to streptomycin associated with three mutant genes, rpoB (27.3) and rrs (27.3 %) and rpsL ( 9.10 %) respectively.
URI: http://hdl.handle.net/123456789/8056
Date: 2015-04-01


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