University of Khartoum

Bacterial Diarrhoea In Infants With Special Reference to The Genus Escherichia In Khartoum State

Bacterial Diarrhoea In Infants With Special Reference to The Genus Escherichia In Khartoum State

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dc.contributor.advisor Elsheikh Ali Elobeid en_US
dc.contributor.author Yagoub, Mohammed
dc.date.accessioned 2015-04-03T13:38:41Z
dc.date.available 2015-04-03T13:38:41Z
dc.date.issued 2015-04-03
dc.date.submitted 2005
dc.identifier.uri http://hdl.handle.net/123456789/8195
dc.description 157 Pages
dc.description.abstract The principal objective of this research was to determine the causative microorganisms of infantile diarrhoea, focusing mainly on Escherichia species, and making comparative study of rest ofbacterial pathogens associated with diarrhoea, as far as their identification and their reaction to different antimicrobial agents of common use. The present work methodology included the isolation, purification, identification and antibiotic sensitivity testing of bacteria which were isolated from infants suffering diarrhoea in different parts of Khartoum State, Sudan. The method adopted for native isolation and identification purposes was the culturing of faeces in differential and selective culture media such as MacConkey’s agar and Eosin Methylene Blue agar using streaking method. The purified isolates were, then, subjected for biochemical activity tests and serotyping. The results obtained revealed that, 234 isolates were collected from the 200 stool samples from infants, which were subjected to a series of purification and identification experiments. All the 234 isolates were Gram-negative rods in shape, and alone E. coliconstituted 141 of all the identified isolates. Of these isolates 34 were classified, using serotyping, as enteropathogenic E. coli, 16 as Enterotoxigenic E. coli and 12 as Enterohaemorrhagic E. coli. The yield of other pathogens was, also, detected and subjected to identification tests. The tests revealed 23 isolates ofProteus, 14 isolates of Shigella spp., and 13 isolates of each of Salmonella, Klebsiella, Pseudomonas. Also Citrobacterwas represented in 12 samples in this study. Morganellawas isolated from 4 samples. Using the disc diffusion technique the clinical strains of E. coli (141), Proteus mirabilis(23), Shigella(14), Salmonella(13), Klebsiella pneumoniae (13), Pseudomonasaeruginosa (13), Citrobacter freundii(12), Morganella morganii(4), Enterobacter(1) were subjected to susceptibility tests to different antibiotics. The results of these tests indicated that many strains exhibited multiple drug resistance. On reviewing the contemporary and current literature in this domain of research, a massive widespread of acute infantile diarrhoea was observed especially in less developed countries, where a high resistance of the causative pathogens to antibiotics was evident. Itwas, also, shown that the bacterial infantile diarrhoea infections usually occur as one product of poor personal and community hygiene. en_US
dc.language.iso en en_US
dc.publisher University of Khartoum en_US
dc.subject Genus Escherichia,Bacterial Diarrhoea,Infants,Khartoum State;Endogenous Transmission of Diarrhoea;Diarrhoea Caused by Injurious Factors;Antigens;Toxins ;Enzymes;Fusidic Acid; Culture Media ; Antibiotics ;Sugars ; Chemicals ; Reagents;Instruments ; Equipment ;Test Control Organisms ;Pathogenic E.coli Immune Sera ;Study Population (Infants) ;Study Area ; Methods ;Specimens en_US
dc.title Bacterial Diarrhoea In Infants With Special Reference to The Genus Escherichia In Khartoum State en_US
dc.type Thesis en_US
dc.Degree M.Sc en_US
dc.Faculty Pharmacy en_US
dc.contributor.faculty Pharmaceutical Microbiology en_US

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