University of Khartoum

Susceptibility of Mycobacterium tuberculosis isolates to six local medicinal plants

Susceptibility of Mycobacterium tuberculosis isolates to six local medicinal plants

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dc.contributor.advisor Susceptibility of Mycobacterium tuberculosis isolates en_US
dc.contributor.author Osman, Elmutasim
dc.date.accessioned 2015-04-03T15:21:42Z
dc.date.available 2015-04-03T15:21:42Z
dc.date.issued 2015-04-03
dc.date.submitted 2005-04
dc.identifier.uri http://hdl.handle.net/123456789/8207
dc.description.abstract The investigation was carried out on 130 pulmonary tuberculosis specimens from human, obtained from Abo anga and El shaab hospitals. The number of the infected males was 103, and females 27, mostly between 20 – 30 years. The specimens were directly smeared, fixed and stained with Ziehl-Neelsen. The acid-fast bacilli (AFB) appeared as red, straight or slightly curved rods, singly or in small groups against blue background. The positive results found in 90 (69.23%), and negative in 40 (30.77%). The positive specimens were cultured on Löwenstein-Jensen ‘L.J.’ medium. The study was done on the 15 pure positive mycobacterial growth for their physiological characters and conventional biochemical tests. Seven specimens were identified and studied for their susceptibility to anti-tuberculous drugs used in Sudan. The findings showed resistance to the common used drugs. The drug resistance patterns to SM, RMP, EMB and INH were : 3 (42.86%), 3 (42.86%), 4 (57.14%), 2 (28.57%) respectively. None of them was resistant to all drugs, and 1 (14.29%) was found to be sensitive to the drugs. Mono resistance was in 1 (14.29%), and multi-drug resistance ‘MDR-TB’ were 5 (71.43%), resistant to two or three drugs were 4 (57.14%), 1 (14.29%) respectively. V The seven isolates tested against extracts of some Sudanese medicinal plants that has previously exhibited inhibitory effect against other microorganisms. The most active extracts (7) belonging to 6 plants (7 parts) related to two families showed activity against the clinical isolates, specially four extracts, then, the minimum inhibitory concentrations were determined. These plants that exhibited high anti-TB activity were phytochemically screened. It can be concluded that certain tannins, saponins, flavonoids play major role in anti-TB activity. en_US
dc.language.iso en en_US
dc.publisher UOFK en_US
dc.subject Mycobacterium,tuberculosis,isolates en_US
dc.title Susceptibility of Mycobacterium tuberculosis isolates to six local medicinal plants en_US
dc.type Thesis en_US
dc.Degree M.Sc en_US
dc.Faculty Pharmacy en_US
dc.contributor.faculty Pharmaceutics en_US

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