University of Khartoum

Antibacterial activity of clove Eugenia caryophllata extracts on bacteria isolated from animal and human

Antibacterial activity of clove Eugenia caryophllata extracts on bacteria isolated from animal and human

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Title: Antibacterial activity of clove Eugenia caryophllata extracts on bacteria isolated from animal and human
Author: Ahmed, Osman
Abstract: Eugenia caryophllata is a plant which is believed by Sudanese herbalists to have antimicrobial effect. This plant has been tested in the present study to investigate their in vitro potential effect against nine Gram positive and Gram negative bacterial species. The tested organisms were Staphylococcus aureus, Streptococcus pyogense, Streptococcus pneumoniae, Bacillus cereus, Corynebacterium renale, Pseudomonas spp, Klebsiella spp, E. coli and Salmonella spp. The plant was extracted with three solvents, water, methanol and petroleum ether. Methanol was used to extract the polar compounds and the petroleum ether was used to extract the fatty compounds. The extracts were tested by three methods, agar dilution method, impregnated filter paper disc method and cup plate method. Water, methanol and petroleum ether extracts of the clove were examined at three concentrations, 10%, 20% and 30%. In the disc method and the cup plate method the extract was prepared and examined in three concentrations of 10%, 20% and 30% and the test organisms were cultured on DST medium. Water extract of Eugenia caryophllata inhibited the growth of Staphylococcus aureus, Bacillus cereus, Corynebacterium renale, Klebsiella spp, E.coli, Pseudomonas spp and Salmonella spp at all concentrations. Methanolic extract of Eugenia caryophllata inhibited the growth of Staphylococcus aureus, Bacillus cereus, Corynebacterium renale, Klebsiella spp, E.coli, Pseudomonas spp and Salmonella spp at all concentrations. But the petroleum inhibited the growth of Bacillus cereus, E.coli and Klebsiella spp at high concentration (30%). The three extracts of Eugenia caryophllata examined in this study at different concentrations were not effective against Streptococcus pyogense and Streptococcus pneumoniae. Water extract of Eugenia caryophllata was found to be more effective as antimicrobial agent than the methanolic extract and petroleum ether extract. Methanolic extract of Eugenia caryophllata was found to be more effective as antimicrobial agent than the petroleum ether extract. In the present study Eugenia caryophllata (Clove) was found to be a good antimicrobial agents. More work on Eugenia caryophllata (Clove) may prove it is a powerful antimicrobial agent.
Description: 93 Pages
URI: http://khartoumspace.uofk.edu/handle/123456789/8529
Date: 2015-04-08


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