University of Khartoum

Identification and Antibiotic Susceptibility of Salmonella Isolated from Diarrhoeal Children of Displaced People: Dar Elsalam, Khartoum, Sudan

Identification and Antibiotic Susceptibility of Salmonella Isolated from Diarrhoeal Children of Displaced People: Dar Elsalam, Khartoum, Sudan

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Title: Identification and Antibiotic Susceptibility of Salmonella Isolated from Diarrhoeal Children of Displaced People: Dar Elsalam, Khartoum, Sudan
Author: Deng, Simon
Abstract: In this study, Salmonella associated with infant diarrhoea was studied in a group of 50 children from people who were brought to Dar-assalam camp at the vicinity of Omdurman Province, Khartoum State. Diagnostic procedures (cultural, biochemical and serological) were carried out to optimize detection of Salmonella species in the samples collected (50 stool samples and 20 serum samples). Serum samples were collected from the cases found positive in isolation. Salmonella was isolated from 20 stool samples (40%). Nine cases were positive for S. Typhi, four were positive for S. Paratyphi and seven were positive for S. Typhimurium. Isolation results of S. Typhi and S. Paratyphi were confirmed by serology (Widal test), where sera of cases positive for S. Typhimurium isolation, were negative (insignificant antibody titre). Co-infections were also detected. Most of the primary cultures of the stool samples were mixed cultures. Escherichia coli was isolated from ten and Shigella from four. Also, in the direct wet mounts of stool samples, Amoeba was observed in six, flagellates in five and worms (Scaris and Hymenolepis nana) in two samples. The antibiotic susceptibility test was performed for the 20 Salmonella isolates against eleven antimicrobial agents, namely, ciprofloxacin, cefotaxime, chloramphenicol, kanamycin, gentamycin, ampicillin, streptomycin, nalidixic acid, cephalexin, tetracycline and Septrin® (sulphamethoxazole and trimethoprim). All the isolates were highly sensitive to ciprofloxacin, which was the most potent antibiotic used, followed by cefotaxime. All S. Typhimurium isolates were resistant to chloramphenicol, while S. Typhi and S. Paratyphi isolates showed high to moderate sensitivity to chloramphenicol. All the isolates of the three species were moderately sensitive to Septrin. On the other hand, all the isolates were resistant to other antibiotics. The results of the antibiotic susceptibility showed that the isolates were sensitive only to a few antibiotics and this may indicate emerging resistance to commonly used antibiotics. Therefore, in routine laboratory diagnostic work, beside pathogen isolation and identification, control measures should also include drug-sensitivity test.
URI: http://khartoumspace.uofk.edu/handle/123456789/8589
Date: 2015-04-08


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