University of Khartoum

In vitro Assessment of the Antimicrobial potential of Bee’s Honey on some Human Pathogens

In vitro Assessment of the Antimicrobial potential of Bee’s Honey on some Human Pathogens

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Title: In vitro Assessment of the Antimicrobial potential of Bee’s Honey on some Human Pathogens
Author: MUSA, NADIR
Abstract: Some Sudanese herbalists believed that the honey of Acacia nilotica and Balanites aegyptiaca can cure urinary tract, wound, burn and abscesses infections. The two honey samples have been tested in the present study to investigate their in vitro potential effect against the bacteria with urinary tract, wound, burn and abscesses infections. A total of sixty samples were collected randomly in Omdurman city from patients of different ages and sexes with urinary tract, wound, burn and abscesses infections during the period January to August 2005. The Gram-positive bacteria isolates were Staphylococcus aureus 38.33%, Staphylococcus epidermidis 6.66%, Staphylococcus saprophyticus 1.66%. The Gram-negative bacteria isolates were Escherichia coli 23.33%, Pseudomonas aeruginosa 18.33%, Klebsiella pneumoniae 5%, Enterobacter clocae 3.33% and Proteus mirabilis 3.33%. The types strain bacteria tested for controlling tests were Staphylococcus aureus ATCC25932, Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27873, Klebsiella pneumoniae ATCC35657 and Proteus mirabilis ATCC35659. Antibiotics sensitivity tests were done for each isolates. The majority isolates were sensitive to Gentamicin 81.66% while Chloramphenicol 36.66%,Ampicillin30% and Tetracycline 21.66% where shown less effective than Gentamicin. The antimicrobial substances in Acacia nilotica and Balanites aegyptiaca honey were not estimated except for pH and the P H were estimated as 4.5 and 5.5 respectively. Acacia nilotica and balanites aegyptiaca honey were tested for their antimicrobial activity by cup-plate techniques. Four different concentrations of the two honey samples were examined 8%, 16%, 32%, 64%. The most effective concentration was 64% concentration as 100% of the isolates showed growth inhibition at this concentration while 81.66% at 32% and 46.66% at 16% and 46.66% at 8% concentration of Acacia nilotica honey. In the present study Acacia nilotica honey was found to be effective as inhibited 100% ofisolates bacteria while Balanites aegyptiaca less effective as inhibited 70%of the isolates by 64% concentration. The minimum inhibition concentration of Acacia nilotica honey against types strains and isolates of Staphylococcus aureus was 8% while against Escherichia coli, Pseudomonas aeruginosa, Klebsiella penumoniae, Proteus mirabilis and Enterobacter clocae was 32% by cupplate techniques. Klebsiella pneumoniae and Pseudomonas aeruginosa types strains and isolates showed resistance to four concentrations of Balanites aegyptiaca honey used. In this investigation Acacia niloticahoney is found to be more effective than Balanites aegyptiaca, Gentamicin, Chloramphenicol, Ampicillin and Tetracycline for treatment urinary tract, wound, burn, and abscesses infections in Omdurman city
Description: 103page
URI: http://khartoumspace.uofk.edu/handle/123456789/8668
Date: 2015-04-09


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