University of Khartoum

Safety and Hematinic Efficacy of Grewia tenax (Godem) and Cajanus cajan (Lobia adasi) in Wistar Rats

Safety and Hematinic Efficacy of Grewia tenax (Godem) and Cajanus cajan (Lobia adasi) in Wistar Rats

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Title: Safety and Hematinic Efficacy of Grewia tenax (Godem) and Cajanus cajan (Lobia adasi) in Wistar Rats
Author: Ahmed, Huda Ahmed Abuaraki
Abstract: This study investigates in rats the safety and haematinic efficacy of the two plants Grewia tenaxand Cajanus cajan. These plants are used traditionally in Sudan for the treatment and prevention of iron deficiency anemia. Forty Wistar breed rats of both sexes wereused (6 weeks old), to evaluate the safety and hematinic efficacy of aqueous extracts of either plant.The aqueous extract of the whole fruit was used for Grewia tenax, while for Cajanus cajan the aqueous extract of the seed was employed. For each plant extract experiment, the animals were divided intofour groups, each of ten animals. Group 1 rats served as untreated controls and were given only distilled water. Animals of groups 2, 3 and 4 were treated daily by oral gavage with the aqueous extract at the dose of 10, 15 and 20 gm/kg.b.w. respectively. The animals were given the respective extract daily for a period of 45 days. Then each group was subdivided randomly into two subgroups of five animals (subgroups A and B) of either sex. Those of subgroup B continued to take the extract for another 45 days, while rats of subgroup A were given distilled water for the same period of time. Rats wereweighed on day 0,45 and 90. Blood samples (2 ml) from each experimental rat were obtained on day 0, 45 and 90 in EDTA tubes by bleeding from the ophthalmicvenous plexus (orbital sinus). The collected blood was used for complete blood counts (CBC). Furthermore, blood samples (4 ml) were collected on day 90 at necropsy for the purpose of biochemical analyses. xii The results of this study show that the oral administration of different dose levels of the whole fruit of Grewia tenax or seed of Cajanus cajanaqueous extracts did not produce any toxicological effects. There were no any significant differences between tested animals of various groups in blood parameters (RBC, WBC and blood platelets counts, HCT percentage, MCV, and MCH) or serum biochemical constituents (Iron concentration, total iron binding capacity, total protein, and albumin and the activity of alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase) compared with control groups. So the results confirmed the safety of the two plants aqueous extracts in Wistar breed rats. As normal rats were used in this study, the two plant preparations did not demonstrate hematinic efficacy on blood constituents especially heamoglobin concentration, red blood cells count, iron and iron binding capacity, in spite of their high contents of iron and the presence of vitamin C in the seeds of Cajanus cajan, which enhance iron absorption. Further investigations are needed to be conducted in anemic animals.
Description: 112 Pages
URI: http://khartoumspace.uofk.edu/handle/123456789/8785
Date: 2015-04-09


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