University of Khartoum

The effect of Water quality onthe efficacy of Newcastle Disease Vaccine

The effect of Water quality onthe efficacy of Newcastle Disease Vaccine

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Title: The effect of Water quality onthe efficacy of Newcastle Disease Vaccine
Author: AbdelWahab, Amira
Abstract: In this study, samples of water sources commonly used to prepare Newcastle disease vaccine in poultry were collected for analysis and experimental vaccination trials. Water samples were collected from different areas; artesian well from Omdurman, shallow well from Elsagai, tap water was collected from Shambat, surface water from Elgiraif and commercially bottled water. Water samples were tested to determine the quality of water using the main parameters; pH, conductivity, TDS, TSS, turbidity and salinity. Turbidity results showed great variation between the water sources examined (artesian well, shallow well, tap water, surface water and bottled water) 4, 5, 0, 48 and zero N.T.U respectively for group A, B, C, D and E. TSS analysis resulted in0.8, 1.4, 0, 32 and zero mg\L respectively. P H levels were between 6.6, 7.4, 7.21,7.18 and 7.32 respectively. Conductivity results were 309, 928, 253, 253 and 207 respectively. TDS results were148, 442, 120, 127, and 96 mg\L respectively. Salinity results 0.1 were equal for all groups. A number of 40 chicks in each group were vaccinated on the 14 th day of age with LaSota vaccine of Newcastle Disease prepared in artesian well (group A), shallow well (group B), tap water (group C), surface water (group D) and bottled water (group E). Blood was collected every week till the 5 th week and serum was tested for antibodies (Ab) against ND using hemagglutination inhibition (HI) test to determine the best immune response. Based on the experiment results of the geo mean HI titers for group E (bottled water) was the best group with regards to immune response. From the 1 st to the 5 th week, Ab titers were 5.05, 6.47, 4.39, 3.95, and X 3.52 log2respectively. The second best immune response was that of group C (tap water) with 4.54, 6.38,4.52, 3.95 and 3.63 respectively. Group A gave 2.78, 4.41, 4.39, 4.38 and 3.52 log2respectively. Group B results were 3.52, 4.89,4.28, 3.32 and 3.26 log2respectively. On the other hand, group D results showed great difference from other groups with 2.49, 3.95, 4.94, 3.14 and 2.88 log2respectively. The present study revealed great variations in water quality as measured by pH, conductivity, TDS,TSS and turbidity and in the immune response when these five water sources were used to prepare LaSota vaccine in chickens. It isrecommended after this study to use bottled water or tap water to prepare drinking water vaccines in poultry farms in Khartoum State.
URI: http://khartoumspace.uofk.edu/handle/123456789/8860
Date: 2015-04-11


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