University of Khartoum

Bovine Mastitis Etiological, Clinical and Treatment Aspects in Khartoum State-Sudan

Bovine Mastitis Etiological, Clinical and Treatment Aspects in Khartoum State-Sudan

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Title: Bovine Mastitis Etiological, Clinical and Treatment Aspects in Khartoum State-Sudan
Author: RABIE, REEM
Abstract: This study was conducted in certain area at Khartoum State from (May/2006- April/2008) to determine the causative agent of bovine mastitis and the susceptibility of different isolates to different antibiotics use for treatment of bovine mastitis. The total number of dairy cows, which were examined in 34 investigated farms, equals about 500. The thirty-four dairy farms were examined at the University, Eltebna, Elhalfaia, Elsamrab, Shambat, Hilat kuku and Falasteen farms. Samples were taken according to owner′s complain from apparent clinical cases of mastitis. Hundred milk samples were collected a septically from 41 cows suffering from mastitis. All these cows were examined by visual inspection and palpation of mammarygland and supramammary lymph nodes. The result as follows: 55% acute mastitis, 44% chronic mastitis and 1% gangrenous mastitis. First test was applied in field for pH detection by using indicator paper, in positive reaction the spot was changed from yellow to green or bluish green that means the pH increased dueto decrease of casein and lactose and increase chloride and carbonate. The samples were taken from apparent clinical cases in sterilebottles for culture, isolation and identification of the causative agentsin all cases. The samples were cultured on MacConkey′s agar and Blood agar and incubated at 37°C for 24 hours and then purified by further subculturing. Smears were prepared and stained with Gram´s stain to detect colour, shape and spores if the later was present. The tests were applied as follows: Primary tests: These classified into: 1- Stained smear. 2- Motility. 3- Oxidase. 4- Catalase. 5- Oxidation Fermentation. 6- Glucose fermentation. The isolated genera were as follows: 74% Bacillusspp., 24% Staphylococcusspp., 1% Corynebacteriumspp. and 1% Klebsiellaspp. After that the secondary tests were applied for isolated genera to identify the species, the results were as follows: 31% Bacillus coagulans, 11% B. cereus, 9% B. subtilis, 9% B. licheniformis, 4% B. circulans,2% B. lentus, 3% B. mycoides, 3%B. amyloliquefaciens,2% B. megaterium, 16% Staphylococcus aureus, 8% Staphylococcus hyicus,1% Corynebacterium spp. and 1% Klebsiellaspp. Lastly, the sensitivity test was applied using different antibiotics were as follows: Chloramphenicol, Ciprofloxacin, Gentamycin, Tetracycline, Piperacillin/ Tazobactam, Pefloxacin, Amikacin, Ofloxacin, Co-Trimoxazole, Ceftizoxime, Cefotaxime and Ampicillin/ Sulbactam. The effectiveness of different antibiotics in different isolates were as follows: Hundred percent of isolates were sensitive for Chloramphenicol and Ciprofloxacin, 91.6% for Gentamycin and Piperacillin/ Tazobactam, 83.3% for Pefloxacin and Tetracycline, 75% for Amikacin and Ofloxacin, 66.6% for Ceftizoxime, 33.3% for Co-Trimoxazole and Cefotaxime and 16.6% for Ampicillin/ Sulbactam.
URI: http://khartoumspace.uofk.edu/handle/123456789/8945
Date: 2015-04-14


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