University of Khartoum

Use of AgNOR Method in Oral Dysplasia and Squamous Cell Carcinoma in Toombak and non-Toombak Dippers

Use of AgNOR Method in Oral Dysplasia and Squamous Cell Carcinoma in Toombak and non-Toombak Dippers

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Title: Use of AgNOR Method in Oral Dysplasia and Squamous Cell Carcinoma in Toombak and non-Toombak Dippers
Author: Ahmed, Hind
Abstract: Background: Argyrophilic Nuclear organizer regions (AgNORs) are associated with proliferative activity and stands as a characteristic mark in the diagnosis of several neoplastic entities. Objective: To assess cellular proliferative activity using AgNOR in oral epithelial dysplasia and squamous cell carcinoma in toombak and non-toombak dippers in Sudan. Materials and Methods: The study is a hospital-based analytical correlation study. Sixty two biopsies of oral epithelial dysplasia and squamous cell carcinoma from 54 toombak and non-toombak dipper patients made the sample of this study. Biopsies were collected in labelled sterile containers from KTDH during the period June 2009 to March 2010. The lab work was carried out in the Oral Pathology laboratory, Faculty of Dentistry, University of Khartoum where the biopsy were fixed with neutral buffered formalin then embedded and serially sectioned. The sections were stained using H&E stained and then diagnosed. Other sections were stained with silver nitrate and then mean count of AgNOR obtained. Results: The Mean AgNOR estimated in toombak dippers compared to non-toombak dippers provided statistically significant Please purchase PDFcamp Printer on http://www.verypdf.com/ to remove this watermark. VI information that toombak dipping is associated with less cellular proliferation. Furthermore, the mAgNOR values showed a gradual increase from well differentiated squamous cell carcinoma to moderately differentiated SCC and finally to the poorly differentiated SCC which showed the highest mean. Conclusion: The mean AgNOR count appears to be a useful diagnostic tool to distinguish between different histopathological grades of oral squamous cell carcinoma and can be used as a supportive tool to routinely performed Hemotoxylin and Eosin staining. Further prospective studies with larger number of cases are needed especially for oral epithelial dysplasia to have more significant conclusions.
URI: http://khartoumspace.uofk.edu/handle/123456789/9017
Date: 2015-04-15


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