University of Khartoum

Effects of Bacillus thuringiensisToxins on the Gut Epithelium of Anopheles arabiensisMosquito Larvae

Effects of Bacillus thuringiensisToxins on the Gut Epithelium of Anopheles arabiensisMosquito Larvae

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dc.contributor.advisor Prof. S.M. EL Sanousi en_US
dc.contributor.author Ahmed, Manhal
dc.date.accessioned 2015-04-18T13:30:26Z
dc.date.available 2015-04-18T13:30:26Z
dc.date.issued 2015-04-18
dc.date.submitted 2010-01
dc.identifier.uri http://khartoumspace.uofk.edu/handle/123456789/9081
dc.description.abstract This study was carried out to assess and evaluate the effect of Bacillus metabolites on Anopheles arabiensismosquito larvae as well as to demonstrate the specific sites for reception of the toxin in mosquitoes. Anopheles arabiensis was reared under standard insectary conditions at 28ºC, 80% humidity and 24hours, light period to ensure large population of larvae to use it in this work. Samples were obtained from mid-gut of mosquito and subjected to bacteriological examination. Bacillusspecies were isolated and identified. The isolates showed diverse morphological and biochemical properties among the species and strains belonging to the same species. Further biochemical test showed that the isolates were Bacillus thuringiensis, B. sphaericus, B. larvae, B. popilliae, B .lentimobus, B. lentus, B. cereus, B. polymyxa, B. firmus, B. megaterium, B. circulans, B. laterosporus, B. brevis, B. badius, B. pantothenticus, B. coagulans, B. alvei and B. mycoides. Test for larvicidal activity of their metabolites against Anophelesmosquito larvae produced 75%-100% mortality rate by 3-5 mlof the products. The Bacillusspore-crystal mixture of50-150 µl applied to An. arabiensislarvae caused 80%-100% mortality. The effect ofphysical factors, such as pH, UV light and temperature, on the larvicidal toxicity was tested using An. arabiensis mosquito larvae in the laboratory. The spore-crystal mixture was subjected to amino acid analysis. The data of analysisseemed to be similar, but not identical in all Bacillusspecies. SDS-PAGE profile of Bacillusmetabolites, Bacillus spore-crystal mixture and infected and healthy mid-gut of mosquito larvae demonstrated significant differences in protein composition. Randomly amplified polymorphic DNA (RAPD) was used to discriminate species of Bacillus. An 838bp, 643bp was identified. A phylogenetic tree generated from VI RAPD patterns of the three strains showed that they could be grouped into two major clusters (a ) and (b) according to genetic similarity in their RAPD profiles. A polyclonal antibody of Bacillusspecies was produced in white rabbits and confirmed by Ouchterlony double diffusion technique. The research achieved the location of sites of receptors on the mid-gut of mosquito larvae which were detected by direct and indirect immunofluorescence. The toxin of Bacilluswas safe on rats when orally applied. en_US
dc.language.iso en en_US
dc.publisher UOFK en_US
dc.subject Bacillus, thuringiensis,Toxins,Gut, Epithelium, Anopheles, arabiensis,Mosquito,Larvae en_US
dc.title Effects of Bacillus thuringiensisToxins on the Gut Epithelium of Anopheles arabiensisMosquito Larvae en_US
dc.type Thesis en_US
dc.Degree Ph.D en_US
dc.Faculty Veterinary Medicine en_US
dc.contributor.faculty Microbiology en_US

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