University of Khartoum

The Influences of High Saturated Fats versus High Cholesterol Diets on Rats Blood Cholesterol

The Influences of High Saturated Fats versus High Cholesterol Diets on Rats Blood Cholesterol

Show full item record

Title: The Influences of High Saturated Fats versus High Cholesterol Diets on Rats Blood Cholesterol
Author: Elshafie, Khalid
Abstract: This study was conducted in the department of Biochemistry faculty of Veterinary Medicine University of Khartoum, to evaluate the influences of feeding high saturated fat diet versus feeding high cholesterol diet on blood cholesterol and to detect the presence of atherogenic changes in tissues of heart and Liver in rats. Thirty Wister albino rats were used in this study. The rats were divided into three groups named A, B and C of ten rats each. Group A was given basal diet only and served as control. Group B received the rats basal diet mixed homogeneously with cholesterol powder as 2% of the diet. Group C received saturated fats mixed as 14% of the basal diet. The experimental period extended for three months. Blood samples were collected after the first, second and third month from all groups, to estimate the levels of cholesterol and its fractions together with the triglycerides concentration.Then all rats were scarified and tissues were collected for histopathology. When the three groups were compared together, group B showed significantly (P> 0.05) higher levelsof total cholesterol and LDL-C compared to the control group for all readings. Whereas, VLDL-C and TAG reported significantly (p> 0.05) Lower levels compared to the control group for all readings. The HDL-C showed significantly (P> 0. 05) higher values after the first and third month but after the second month it showed significantly (P> 0.05) Lower value compared to the control group. ix In group C the levels of total cholesterol, LDL–C, HDL-C, VLDL-C and TAG were non–significantly differentfor the three readings, with numerically higher values for the total cholesterol and the LDL-C in all readings compared to the control group. The results obtained from this work, indicated that high dietary cholesterol affect plasma lipid profile concentration while feeding high saturated fats showed non significant changes on lipid profile of the plasma. For the histopathological findings in group B the heart showed edema of cardiac muscles, zenker necrosis, hyalinization and thickness of blood vessels and presence of cholesterol cleft while heart in group C showed hyalinization of cardiac muscles fiber, edema, infiltration of RBCs in the heart muscle, blood vessels thickness and mild monocular cellular infiltration with zenker necrosis. In group B, the liver showed vacuolization , dilatation of sinusoid, necrosis and disorganization of hepatocytes .while liver in group C showed vacuolization of hepatic cytoplasm, congestions of veins, of sinusoids, bile duct proliferation, kuffer cells were prominent, congestion of central veins and extra hepatic hemopoisis and focal infiltration of inflammatory cells and necrosis. So histopathological findings indicated that both high dietary cholesterol and high dietary saturated fats showed clear histopathological changes in tissues of the heart and liver. The histopathological changes observed for both treated groups, recorded more signs of inflammation in the group received high saturated fat diet. The control showed healthy histopathology tissues. The present study concluded thatthough high cholesterol diet significantly influenced the blood cholesterol and triglyceride levels, x whereas histopathological changes showed that both high cholesterol diet and high saturated fat dietresulted in clear changes to both treated groups with more inflammatory signs ingroup received saturated fats. So future studies includes more number of animals and to be subjected to more experimental period may give clear picture about the actual effect of both treatments also other atherogenic indices should be measured to evaluate which is the most responsible of inducing atherosclerosis is it the high cholesterol diet or the high saturated fat diet.
Description: 88page
URI: http://khartoumspace.uofk.edu/handle/123456789/9089
Date: 2015-04-18


Files in this item

Files Size Format View

This item appears in the following Collection(s)

Show full item record

Share

Search DSpace


Browse

My Account